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Deinococcus aestuarii sp. nov. and Deinococcus aquaedulcis sp. nov., two novel resistant bacteria isolated from pearl river estuary

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Abstract

Two novel species of the genus Deinococcus, designated SYSU M49105T and SYSU M42101T, were isolated from freshwater samples of the Pearl River estuary in Guangdong, China. Phylogenetic analysis using 16S rRNA gene sequence indicated that strains SYSU M49105T and SYSU M42101T showed the highest sequence similarities to Deinococcus aetherius JCM 11751 T (93.6%) and Deinococcus multiflagellatus NBRC 112888 T (97.3%), respectively. Cells of both strains were Gram-staining positive, aerobic, coccus-shaped, oxidase-negative and non-motile. The cell wall contained meso-diaminopimelic acid as their diagnostic diamino acid. MK-8 was the predominant respiratory quinone for both strains. The polar lipid profile of SYSU M49105T contained two unidentified phosphoglycolipids, nine unidentified glycolipids, and five unidentified polar lipids. SYSU M42101T had one unidentified phosphoglycolipid, nine unidentified glycolipids, one unidentified aminophospholipid and four unidentified polar lipids. The major fatty acids of strains SYSU M49105T and SYSU M42101T were summed feature 3 (C16:1 ω7c and/ or C16:1 ω6c) and C16:0. The G + C contents of the novel isolates based on genomic DNAs were 69.6% and 67.4%, respectively. On the basis of phenotypic, genotypic and phylogenetic data, strains SYSU M49105T and SYSU M42101T should be considered to represent two novel species in the genus Deinococcus, for which the names Deinococcus aestuarii sp. nov. and Deinococcus aquaedulcis sp. nov. were proposed with the type strains SYSU M49105T (= KCTC 43258 T = CGMCC 1.18609 T) and SYSU M42101T (= KCTC 43257 T = CGMCC 1.18614 T), respectively.

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Availability of data and material

The 16S rRNA gene sequences of strains SYSU M49105T and SYSU M42101T have been deposited in GenBank under the accession numbers MZ496817 and MZ501833, respectively. Whole Genome Shotgun projects of strains SYSU M49105T and SYSU M42101T have been deposited at DDBJ/ENA/GenBank under accession numbers JAHKSN000000000 and JAHRBL000000000, respectively. The genome raw data of strains SYSU M49105T and SYSU M42101T have been deposited in GenBank under the accession numbers PRJNA662819 and PRJNA733564, respectively. Five supplementary tables and four supplementary figures are included on the online supplementary files.

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Acknowledgements

The authors are grateful to Prof. Aharon Oren (The Hebrew University of Jerusalem, Israel) for helping with the etymology.

Funding

This work was financially supported by National Science and Technology Fundamental Resources Investigation Program of China (2021FY100909), the China Postdoctoral Science Foundation (Nos. 2018M643294, 2020M683029), Guangdong Basic and Applied Basic Research Foundation, China (No. 2020A1515011139), Fundamental Research Funds for the Central Universities, China (Grant No. 19lgpy200) and Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (Nos. 311020003, 311021004, 311021006).

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Authors and Affiliations

  1. State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    Ling-Zi Yin, Jia-Ling Li, Ze-Tao Liu, Pandeng Wang, Xiao-Qing Luo, Lei Dong, Li Duan, Shan-Hui Li & Wen-Jun Li

  2. State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, People’s Republic of China

    Bao-Zhu Fang & Wen-Jun Li

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  1. Ling-Zi Yin
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Contributions

WJL designed research and project outline. ZTL obtained the Pearl River estuary water sample. LZY, LD, XQL, JLL and SHL performed isolation, deposition and polyphasic taxonomy. PDW, LD performed genome analysis. LZY, BZF and WJL drafted the manuscript. All authors read and approved the final manuscript.

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Correspondence to Wen-Jun Li.

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Yin, LZ., Li, JL., Liu, ZT. et al. Deinococcus aestuarii sp. nov. and Deinococcus aquaedulcis sp. nov., two novel resistant bacteria isolated from pearl river estuary. Antonie van Leeuwenhoek 115, 59–68 (2022). https://doi.org/10.1007/s10482-021-01680-x

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