Abstract
A Gram-stain-negative, light yellow pigmented, non-motile and aerobic bacterial strain, designated HHU E2-1 T, was isolated from a surface seawater sample. The 16S rRNA gene sequence analysis indicated that HHU E2-1 T shared the highest sequence similarity to the type strain Qipengyuania gaetbuli DSM 16225 T (96.90%), which belongs to the family Erythrobacteraceae. Combined phylogeny of 288 single-copy orthologous gene clusters, analysis of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH), average amino acid identity (AAI) and evolutionary distances suggested that HHU E2-1 T can be considered as a member of the genus Altererythrobacter based on the recently proposed standard for defining genera of Erythrobacteraceae. Strain HHU E2-1 T grew at 15–35 °C and pH 5.0–8.0, with optimum growth at 28 °C and pH 7.0. Tolerance to NaCl was up to 4% (w/v) with optimum growth in 2–3% NaCl. The major fatty acids (> 10%) were C18:1ω7c11-methyl, summed feature 3 (C16:1ω7c and/or C16:1ω6c), and summed feature 8 (C18:1ω7c and/or C18:1ω6c). The predominant isoprenoid quinone was ubiquinone-10. The genomic G + C content was 57.40%. On the basis of the phenotypic, phylogenetic and chemotaxonomic characterizations, HHU E2-1 T represents a novel species of the genus Altererythrobacter, for which the name Altererythrobacter flava sp. nov. is proposed. The type strain is HHU E2-1 T (= CGMCC 1.17394 T = KCTC 72835 T = MCCC 1K04226T).
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Data availability statement
Most of the data supporting the conclusions of this article are included within the article and its Additional files. The genome datasets and the phylogenetic data of Altererythrobacter flava HHU E2-1 T generated during the current study are available in the GenBank repository under accession number JADCLP000000000 and MW172822, respectively. Other datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
Change history
07 December 2021
A Correction to this paper has been published: https://doi.org/10.1007/s10482-021-01692-7
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Acknowledgements
This work was supported by the National Nature Science Foundation of China (31960001), Yunnan Provincial Science and Technology Project (2017FF116 (-002)), Jiangsu Specially-Appointed Professor Program (JSAPP2016), and the Top Young Talents of Ten Thousand Program in Yunnan province. The authors were also grateful to Dr. Lin Xu (Zhejiang Sci-Tech University) for providing data of the 288 orthologous clusters (OCs) for 74 Erythrobacteraceae genomes.
Funding
This work was supported by the National Nature Science Foundation of China (31960001), Yunnan Provincial Science and Technology Project (2017FF116 (-002)), Jiangsu Specially-Appointed Professor Program (JSAPP2016), and the Top Young Talents of Ten Thousand Program in Yunnan province. Expenditures of sampling are funded by project NO. 31960001; expenditures of sequencing and polyphasic taxonomic experiences are funded by project NO. JSAPP2016 and the Ten Thousand Program; expenditures of student service fees are funded by project NO. 2017FF116 (-002).
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XMZ and YLZ designed research and project outline. XMZ and DFZ performed isolation, deposition and polyphasic taxonomy. DFZ performed genome analysis. DFZ and XMZ drafted the manuscript. YLZ revised the manuscript. All authors read and approved the final manuscript.
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Zhang, XM., Zhang, DF. & Zhang, YL. Altererythrobacter flava sp. nov., a new member of the family Erythrobacteraceae, isolated from a surface seawater sample. Antonie van Leeuwenhoek 114, 497–506 (2021). https://doi.org/10.1007/s10482-021-01531-9
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DOI: https://doi.org/10.1007/s10482-021-01531-9