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FSH1 overexpression triggers apoptosis in Saccharomyces cerevisiae

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Abstract

FSH1 belongs to the family of serine hydrolases in yeast and is homologous to the human ovarian tumor suppressor gene (OVAC2). Our preliminary results showed that cells lacking Fsh1p exhibit an increase in cell growth, and a decrease in the expression of AIF1 and NUC1 (apoptosis responsive genes) when compared to the wild type cells. Growth inhibition of cells overexpressing FSH1 is due to induction of cell death associated with cell death markers typical of mammalian apoptosis namely DNA fragmentation, phosphatidylserine externalization, ROS accumulation, Cytochrome c release, and altered mitochondrial membrane potential. When wild type cells were overexpressed with FSH1 there was up regulation of AIF1 level when compared to control cells suggesting that overexpression of FSH1 regulated cell death in yeast.

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Abbreviations

FSH:

Family of serine hydrolase

S. cerevisiae :

Saccharomyces cerevisiae

MMP:

Mitochondrial membrane potential

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Acknowledgements

We thank Prof. Ram Rajasekharan (Central Food Technological Research Institute, Mysore, India) for providing yeast strains and reagents. We thank the infrastructure facilities from DST-FIST, Department of Biochemistry, Life Sciences & DST-PURSE facilities, of Bharathidasan University.

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Authors and Affiliations

  1. Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620024, India

    Ramachandran Gowsalya, Chidambaram Ravi, Mathivanan Arul & Vasanthi Nachiappan

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  1. Ramachandran Gowsalya
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  2. Chidambaram Ravi
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  3. Mathivanan Arul
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  4. Vasanthi Nachiappan
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Contributions

VN and RG designed the experiments, RG, CR and MA performed the experiments.VN and RG wrote the manuscript. All the authors discussed the results and concluded the manuscript.

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Correspondence to Vasanthi Nachiappan.

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Gowsalya, R., Ravi, C., Arul, M. et al. FSH1 overexpression triggers apoptosis in Saccharomyces cerevisiae. Antonie van Leeuwenhoek 112, 1775–1784 (2019). https://doi.org/10.1007/s10482-019-01310-7

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