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Cellulomonas aurantiaca sp. nov., isolated from a soil sample from a tangerine field

  • Su-Kyung Kim
  • MooChang Kook
  • Zheng-Fei Yan
  • Huan Trinh
  • Sheng-Dao Zheng
  • Jung-Eun Yang
  • Sang-Yong Park
  • Tae-Hoo YiEmail author
Original Paper

Abstract

A Gram-stain positive, facultatively aerobic, motile and rod-shaped bacterial strain, designated THG-SMD2.3T, was isolated from a soil sample collected in a tangerine field, Republic of Korea. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Cellulomonas and to be closely related to Cellulomonas fimi ATCC 484T (98.5%), Cellulomonas biazotea DSM 20112T (98.3%), Cellulomonas chitinilytica X.bu-bT (98.0%), Cellulomonas xylanilytica XIL11T (97.2%), Cellulomonas humilata ATCC 25174T (97.1%) and Cellulomonas composti TR7-06T (97.0%). The 16S rRNA gene sequence similarities with other current species of the genus Cellulomonas were in the range 95.4–96.6%. Catalase and oxidase tests were found to be positive. The DNA G+C content was determined to be 73.0 mol%. DNA-DNA hybridization values between strain THG-SMD2.3T and C. fimi ATCC 484T, C. biazotea DSM 20112T, C. chitinilytica X.bu-bT, C. xylanilytica XIL11T, C. humilata ATCC 25174T and C. composti TR7-06T were 58.1 ± 1.6%, 56.7 ± 0.8%, 30.3 ± 1.6%, 22.8 ± 1.6%, 19.9 ± 1.6%, and 13.5 ± 3.0%, respectively. Strain THG-SMD2.3T was also found to be able to grow at 20–42 °C, at 0–3% NaCl and at pH 5.5–10. The major fatty acids were identified as anteiso-C15:0, iso-C15:0, anteiso-C17:0 and iso-C14:0. The predominant menaquinone was identified as tetrahydrogenated menaquinones with nine isoprene units [MK-9(H4)]. The polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and two unidentified phospholipids. Based on these phenotypic, genotypic and phylogenetic characterisations strain THG-SMD2.3T (= KACC 19341T = CGMCC 1.16303T) is concluded to represent a novel species of the genus Cellulomonas, for which the name Cellulomonas aurantiaca sp. nov. is proposed.

Keywords

THG-SMD2.3 Taxanomy Cellulomonas aurantiaca Tangerine field 

Abbreviations

DPG

Diphosphatidylglycerol

PG

Phosphatidylglycerol

PE

Phosphatidylethanolamine

PL

Unidentified phospholipids

AL

Unidentified aminophospholipid

GL

Unidentified glycolipid

L

Unidentified lipid

Notes

Funding

This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ012655042018)” Rural Development Administration, Republic of Korea.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no direct or indirect conflict of interest.

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

10482_2019_1288_MOESM1_ESM.pptx (775 kb)
Supplementary material 1 (PPTX 775 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Su-Kyung Kim
    • 1
  • MooChang Kook
    • 2
  • Zheng-Fei Yan
    • 3
  • Huan Trinh
    • 1
  • Sheng-Dao Zheng
    • 1
  • Jung-Eun Yang
    • 4
  • Sang-Yong Park
    • 4
  • Tae-Hoo Yi
    • 1
    Email author
  1. 1.Department of Oriental Medicinal Material and Processing, College of Life ScienceKyung Hee University Global CampusYongin-siRepublic of Korea
  2. 2.Department of Food and NutritionBaewha Women’s UniversitySeoulRepublic of Korea
  3. 3.State Key Laboratory of Food Science and Technology, School of BiotechnologyJiangnan UniversityWuxiPeople’s Republic of China
  4. 4.SD Biotechnologies Co., Ltd, Seoul Hightech Venture CenterSeoulRepublic of Korea

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