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Mesorhizobium carbonis sp. nov., isolated from coal bed water

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Abstract

A Gram-stain negative, aerobic, motile, short rod-shaped bacterium, designated as B2.3T, was isolated from coal bed water collected from Jincheng, Shanxi Province, China. The strain was able to grow at 10–40 °C (optimum 28–30 °C), pH 4.0–10.0 (optimum 7.0), and in the presence of 0–5.0% NaCl (optimum 3.0%, w/v). Phylogenetic analysis based on the 16S rRNA and concatenated housekeeping gene recA, atpD and glnA sequences showed strain B2.3T belongs to the genus Mesorhizobium, with Mesorhizobium oceanicum B7T as the closely related type strain. Strain B2.3T exhibited ANI value of 77.5% and GGDC value of 21.5% to M. oceanicum B7T. The major fatty acids were identified as summed feature 8 (C18:1ω7c and/or C18:1ω6c) and 11-methyl C18:1ω7c. The major polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unidentified aminophospholipid. The predominant ubiquinone was identified as Quinone 10. Phenotypic and biochemical analysis results indicated that strain B2.3T can be distinguished from closely related type strains. On the basis of phenotypic, genotypic and chemotaxonomic characteristics, strain B2.3T is concluded to represent a novel species in the genus Mesorhizobium, for which the name Mesorhizobium carbonis sp. nov. is proposed. The type strain is B2.3T (=CGMCC 1.15730T = KCTC 52461T).

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Abbreviations

ANI:

Average nucleotide identity

DDH:

DNA–DNA hybridization

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Acknowledgements

This work was financially supported by the National Natural Science Foundation (30901108 and 21776310), the Shandong Provincial Natural Science Foundation (ZR2009EQ007 and ZR2017MB019) and the Fundamental Research Funds for the Central Universities (15CX02014A and 16CX02044A).

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  1. Wen Xin and Zhen-Zhen Xu have contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, College of Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, People’s Republic of China

    Jing Li, Wen Xin, Zhen-Zhen Xu, Fang-Qin Xiang, Jing-Jing Zhang, Li-Jun Xi, Jian-Bo Qu & Jian-Guo Liu

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Contributions

JL designed the study and wrote the manuscript. WX, ZZX and FQX performed the experiments. JL and LJX analyzed the data. JJZ contributed to the polar lipid analysis. JBQ and JGL revised the manuscript.

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Correspondence to Jing Li or Jian-Guo Liu.

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Li, J., Xin, W., Xu, ZZ. et al. Mesorhizobium carbonis sp. nov., isolated from coal bed water. Antonie van Leeuwenhoek 112, 1221–1229 (2019). https://doi.org/10.1007/s10482-019-01254-y

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