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Mesorhizobium zhangyense sp. nov., isolated from wild Thermopsis lanceolate in northwestern China

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Abstract

A Gram-stain-negative strain, 23-3-2T, was isolated from a nodule of Thermopsis lanceolate grown in Northwest China. Phylogenetic analysis of 16S rRNA gene sequence showed that the strain was closely related to Mesorhizobium camelthorni CCNWXJ 40-4T and M. alhagi CCNWXJ 12-2T having 98.0 and 97.9% similarities, respectively. Phylogenetic analysis based on the protein-coding genes atpD and glnA showed lower similarity with the same closely related species (94.5 and 89.9%, respectively), which suggest that 23-3-2T strain represents a distinctly delineated genospecies of the genus Mesorhizobium. The 23-3-2T strain grew at 20–37 °C temperature (optimum 28 °C) and 5.0–9.0 pH range (optimum pH 7.0). The cells contained Q-10 as the sole respiratory quinone and 18:1ω7c (24.56%) as the major cellular fatty acid. The DNA relatedness between the strain 23-3-2T and the two reference strains was 39–44%. Based on the phenotypic, chemotaxonomic and phylogenetic properties, strain 23-3-2T represents a novel species of the genus Mesorhizobium, for which the name Mesorhizobium zhangyense sp. nov. is proposed. The type strain is 23-3-2T (= CGMCC 1.15528T = NBRC 112337T). The respective DPD Taxon Number is TA00147.

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Acknowledgements

This work was supported by projects from the National Science Foundation of China (31360004), Chinese Scholarship Council and project of Education Department of Gansu Province (2014A-107). We are grateful to Dr. Yuguang Zhou and Lei Song for deposition of the strain in the culture collections. We also thank Professor Gehong Wei and Dr. Weimin Chen for offering the reference strains to us.

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Authors and Affiliations

  1. Key Laboratory of Hexi Corridor Resources Utilization, Hexi University, Zhangye, 734000, Gansu, China

    Lin Xu & Yong Zhang

  2. Department of Biology, University of York, York, YO10 5DD, UK

    Lin Xu & Ville-Petri Friman

  3. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Lin Xu & Chenying Jiang

  4. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences(CAS), Ürümqi, 830011, China

    Osama Abdalla Mohamad

  5. Institute for Post Graduate Environmental Studies, Environmental Science Department, Arish University, North Sinai, 45511, Egypt

    Osama Abdalla Mohamad

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  1. Lin Xu
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  2. Yong Zhang
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  3. Osama Abdalla Mohamad
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Correspondence to Lin Xu or Ville-Petri Friman.

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Communicated by Erko Stackebrandt.

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Xu, L., Zhang, Y., Mohamad, O.A. et al. Mesorhizobium zhangyense sp. nov., isolated from wild Thermopsis lanceolate in northwestern China. Arch Microbiol 200, 603–610 (2018). https://doi.org/10.1007/s00203-017-1464-0

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  • DOI: https://doi.org/10.1007/s00203-017-1464-0

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