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Deinococcus knuensis sp. nov., a bacterium isolated from river water

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Abstract

Strain 16F3HT, a Gram-negative, aerobic, non-motile, and oval-shaped bacterium, was isolated from river water collected from the Han River in South Korea. Growth of strain 16F3HT was observed at 10–42 °C (optimum at 25–30 °C), but no growth occurred at 4 °C. The strain is able to grow at pH 4–10 (optimum at pH 7–8) and tolerates up to 4% NaCl (w/v), with optimum growth at 0.5% NaCl. The isolate was found to be resistant to UV irradiation. Based on 16S rRNA gene sequence analysis, it is closely related to ‘Deinococcus seoulensis’ 16F1E (98.8%), Deinococcus aquaticus PB314T (98.1%) and Deinococcus caeni Ho-08T (98.0%). The level of DNA–DNA homology between the novel strain and the three related strains was 57.4, 41.2, and 35.8%, respectively. Chemotaxonomic data revealed that strain 16F3HT possesses MK-8 as the predominant respiratory quinone, an unidentified phosphoglycolipid as the major polar lipid, and C15:1 ω6c and C16:1 ω7c as the major fatty acids. The DNA G + C content was determined to be 65.7 mol%. Based on polyphasic evidence, strain 16F3HT (=KCTC 33794T = JCM 31406T) should be classified as the type strain of a novel Deinococcus species, for which the name Deinococcus knuensis sp. nov. is proposed.

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Acknowledgements

This work was supported by the Human Resource Training Program for Regional Innovation and Creativity through the Ministry of Education and National Research Foundation (NRF) of Korea (NRF-2014H1C1A1066929), and by the Brain Pool Program of 2016 funded by the Ministry of Science, Republic of Korea (Grant 162S-4-3-1727).

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Authors and Affiliations

  1. School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea

    Jae-Jin Lee, Yeon-Hee Lee, Su-Jin Park, Seung-Yeol Lee, Sangkyu Park, Leonid N. Ten & Hee-Young Jung

  2. Department of Environmental Engineering, Kyungpook National University, Daegu, 41566, Republic of Korea

    Dae Sung Lee

  3. Department of Horticultural Science, Kyungpook National University, Daegu, 41566, Republic of Korea

    In-Kyu Kang

  4. Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea

    Hee-Young Jung

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  1. Jae-Jin Lee
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Correspondence to Hee-Young Jung.

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Lee, JJ., Lee, YH., Park, SJ. et al. Deinococcus knuensis sp. nov., a bacterium isolated from river water. Antonie van Leeuwenhoek 110, 407–414 (2017). https://doi.org/10.1007/s10482-016-0813-3

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