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An endophytic Streptomyces sp. strain DHV3-2 from diseased root as a potential biocontrol agent against Verticillium dahliae and growth elicitor in tomato (Solanum lycopersicum)

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Abstract

Plant endophytes play important roles in biocontrol of plant diseases. Actinomycetes are used for biocontrol of fungal diseases caused by Verticillium dahliae. Many studies have focused on the endophytic actinomycetes isolated from the roots of healthy plants, but few on those from the roots of diseased plants. In the present research, actinomycetes were isolated from the roots of diseased and healthy tomato plants, respectively. The results showed that, in total, 86 endophytic actinomycetes were isolated for screening of their antimicrobial activities, 8 of which showed antagonism to V. dahliae in vitro. Among the 8 antagonistic strains, 5 (out of 36) were from the roots of diseased plants, with inhibition diameter zones ranging from 11.2 to 18.2 mm, whereas 3 (out of 50) were from the roots of healthy plants, with inhibition diameter zones ranging from 11.5 to 15.5 mm. Endophytic strain DHV3-2 was isolated from the root of a diseased plant and demonstrated a potent effect against V. dahliae and other pathogenic fungi by showing the largest inhibition diameter zones among all the eight antagonistic strains. Thus, strain DHV3-2 was chosen to investigate its biological control efficacies in vivo. Further study showed that the disease incidence and disease severity indices of tomato Verticillium wilt decreased significantly (P < 0.05). We also found that the plant shoot fresh weight and height increased greatly (P < 0.05) upon treatment with strain DHV3-2 compared to the plants uninoculated in greenhouse conditions. Root colonization showed that strain DHV3-2 had the higher root-colonizing capacity in the roots of infected plants compared with the roots of healthy plants. This isolate was identified as Streptomyces sp. based on morphological characteristics and 16S rRNA gene analysis. In conclusion, the roots of diseased tomato plants are a potential reservoir of biological control actinomycetes, and Streptomyces sp. strain DHV3-2 is a potential biocontrol agent against V. dahliae and growth elicitor in tomato.

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Acknowledgments

We gratefully acknowledge the National Nature Science Foundation of China (31471917) and Science and Technology Research Project of Heilongjiang Province Department of Education, China (12541859), for their funding of this project.

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    Authors and Affiliations

    1. College of Horticulture, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

      Peng Cao, Xuepeng Fu, Shaoxian Wang & Fengzhi Wu

    2. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

      Chongxi Liu, Pengyu Sun & Xiangjing Wang

    3. Heilongjiang Provincial Key University Laboratory of Cold Area Vegetable Biology, Northeast Agricultural University, Mucai 59, Xiangfang, Harbin, 150030, People’s Republic of China

      Fengzhi Wu

    4. Ministry of Agriculture Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in Northeast China, Northeast Agricultural University, Mucai 59, Xiangfang, Harbin, 150030, People’s Republic of China

      Fengzhi Wu

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    1. Peng Cao
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    2. Chongxi Liu
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    Correspondence to Fengzhi Wu or Xiangjing Wang.

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    Peng Cao, Chongxi Liu and Pengyu Sun contributed equally to this work.

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    Cao, P., Liu, C., Sun, P. et al. An endophytic Streptomyces sp. strain DHV3-2 from diseased root as a potential biocontrol agent against Verticillium dahliae and growth elicitor in tomato (Solanum lycopersicum). Antonie van Leeuwenhoek 109, 1573–1582 (2016). https://doi.org/10.1007/s10482-016-0758-6

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