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Cell wall glycopolymers of Streptomyces albus, Streptomyces albidoflavus and Streptomyces pathocidini

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Abstract

The cell wall glycopolymers of three strains of Streptomyces albus and the type strain of Streptomyces pathocidini were investigated. The structures of the glycopolymers were established using a combination of chemical and NMR spectroscopic methods. The cell wall of S. albus subsp. albus VKM Ac-35T was found to be comprised of three glycopolymers, viz. unsubstituted 1,5-poly(ribitol phosphate), 1,3-poly(glycerol phosphate) substituted with β-d-glucopyranose, and the major polymer, a 3-deoxy-d-glycero-d-galacto-non-2-ulosonic acid (Kdn)-teichulosonic acid: β-d-Glcp-(1 → 8)-α-Kdnp-(2[(→6)-β-d-Glcp-(1 → 8)-α-Kdnp-(2 →] n 6)-β-d-Glcp-(1 → 8)-β-Kdnp-(2-OH, where n ≥ 3. The cell walls of ‘S. albus’ J1074 and ‘S. albus’ R1-100 were found to contain three glycopolymers of identical structures, viz. unsubstituted 1,3- and 2,3-poly(glycerol phosphates), and the major polymer, a Kdn-teichulosonic acid with an unusual structure that has not been previously described: β-d-Galp-(1 → 9)-α-Kdnp-(2[(→3)-β-d-Galp-(1 → 9)-α-Kdnp-(2 →] n 3)-β-d-Galp-(1 → 9)-β-Kdnp-(2-OH, where n ~ 7–8. The cell wall of S. pathocidini (formerly S. albus subsp. pathocidicus) VKM Ac-598T was found to contain two glycopolymers, viz. 1,3-poly(glycerol phosphate) partially O-glycosylated with 2-acetamido-2-deoxy-α-d-glucopyranose and/or O-acylated with l-lysine, and a poly(diglycosyl 1-phosphate) of hitherto unknown structure: -6)-α-d-Glcp-(1 → 6)-α-d-GlcpNAc-(1-P-.

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Acknowledgments

This work was supported by Grant 13-04-00447 from the Russian Foundation for Basic Research and programs of the Presidium of the Russian Academy of Sciences “Molecular and Cell Biology”, and the Ministry of Education and Science of the Russian Federation (Contract No. 16.518.11.7035).

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Authors and Affiliations

  1. ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 47, Moscow, Russia, 119991

    Alexander S. Shashkov, Sophia N. Senchenkova & Andrey S. Dmitrenok

  2. School of Biology MV, Lomonosov Moscow State University, Vorob’evy gory 1 Stroenie 12, Moscow, Russia, 119991

    Galina M. Streshinskaya & Elena M. Tul’skaya

  3. All-Russian Collection of Microorganisms (VKM), G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russia, 142290

    Lidia M. Baryshnikova

  4. Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, Lviv, 79005, Ukraine

    Bohdan E. Ostash & Victor A. Fedorenko

Authors
  1. Alexander S. Shashkov
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  2. Galina M. Streshinskaya
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  3. Elena M. Tul’skaya
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  4. Sophia N. Senchenkova
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  5. Lidia M. Baryshnikova
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  6. Andrey S. Dmitrenok
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  7. Bohdan E. Ostash
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  8. Victor A. Fedorenko
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Correspondence to Galina M. Streshinskaya.

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Shashkov, A.S., Streshinskaya, G.M., Tul’skaya, E.M. et al. Cell wall glycopolymers of Streptomyces albus, Streptomyces albidoflavus and Streptomyces pathocidini . Antonie van Leeuwenhoek 109, 923–936 (2016). https://doi.org/10.1007/s10482-016-0691-8

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