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Lentibacillus amyloliquefaciens sp. nov., a halophilic bacterium isolated from saline sediment sample

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Abstract

A Gram-stain positive, non-motile, non-sporogenous, aerobic, rod-shaped and halophilic bacterium, designated LAM0015T, was isolated from a saline sediment sample collected from Yantai City in China. The isolate was found to be able to grow at NaCl concentrations of 5–25 % (w/v) (optimum: 7–12 %), 15–45 °C (optimum: 35 °C) and pH 5.0–9.0 (optimum: 7.0). The major fatty acids were determined to be anteiso-C15:0 and anteiso-C17:0. The predominant respiratory quinone was identified as MK-7. The cell wall peptidoglycan was determined to contain meso-diaminopimelic acid. The polar lipids were found to be diphosphatidyglycerol, phosphatidylglycerol, five phospholipids and one glycolipid. The DNA G+C content was 43.1 mol% as determined by the T m method. Analysis of the 16S rRNA gene sequence indicated that the isolate belongs within the genus Lentibacillus and is closely related to Lentibacillus persicus DSM 22530T, Lentibacillus salicampi JCM 11462T and Lentibacillus jeotgali JCM 15795T with 97.3, 96.7 and 96.4 % sequence similarity, respectively. The DNA–DNA hybridization value between LAM0015T and L. persicus DSM 22530T was 51.2 ± 1.4 %. Based on its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM0015T is concluded to represent a novel species of the genus Lentibacillus, for which the name Lentibacillus amyloliquefaciens sp. nov. is proposed. The type strain is LAM0015T (=ACCC 06401T = JCM 19838T).

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Acknowledgments

We are grateful to Professor Min Wu from Zhejiang University for his technical assistance. This work was supported by Open Funds of Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin (Tarim University, BRZD1403), National Nonprofit Institute Research Grant of CAAS (No. 2014-30), National Key Technology R&D Program of China (No. 2013BAD05B04F02).

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    Authors and Affiliations

    1. Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture), Biogas Institute of Ministry of Agriculture, Chengdu, 610041, People’s Republic of China

      Jing-Li Wang, Yan-Wei Wang, Xiang Guo & Ming-Xiong He

    2. College of Environmental and Chemical Engineering, Dalian University, Dalian, 116622, People’s Republic of China

      Ke-Dong Ma

    3. Key Laboratory of Microbial Resources (Ministry of Agriculture, China), Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, People’s Republic of China

      Hui-Min Wang, Shan Zhou, Xiao-Rong Chen, De-Long Kong & Zhi-Yong Ruan

    4. College of Life Sciences, Tarim University, and Xinjiang Production & Construction Corps Key Laboratory of Protection and Utilization of Biological Resources, Tarim Basin, Alaer City, 843300, People’s Republic of China

      Yan-Bin Li

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    1. Jing-Li Wang
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    Correspondence to Ming-Xiong He or Zhi-Yong Ruan.

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    Jing-Li Wang and Ke-Dong Ma have contributed equally to the work and share first authorship.

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    Wang, JL., Ma, KD., Wang, YW. et al. Lentibacillus amyloliquefaciens sp. nov., a halophilic bacterium isolated from saline sediment sample. Antonie van Leeuwenhoek 109, 171–178 (2016). https://doi.org/10.1007/s10482-015-0618-9

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    • DOI: https://doi.org/10.1007/s10482-015-0618-9

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