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Characterization of antimicrobial resistance in Salmonella enterica strains isolated from Brazilian poultry production

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Abstract

Antimicrobial resistance profiles and presence of resistance determinants and integrons were evaluated in Salmonella enterica strains from Brazilian poultry. The analysis of 203 isolates showed that those from the poultry environment (88 isolates) were significantly more resistant to antimicrobials than isolates from other sources, particularly those isolated from poultry by-product meal (106 isolates). Thirty-seven isolates were resistant to at least three antimicrobial classes. Class 1 integrons were detected in 26 isolates, and the analysis of the variable region between the 5′ conserved segment (CS) and 3′ CS of each class 1 integron-positive isolate showed that 13 contained a typical 3′ CS and 14 contained an atypical 3′ CS. One Salmonella Senftenberg isolate harbored two class 1 integrons, showing both typical and atypical 3′ CSs. The highest percentage of resistance was found to sulfonamides, and sul genes were detected in the majority of the resistant isolates. Aminoglycoside resistance was detected in 50 isolates, and aadA and aadB were present in 28 and 32 isolates, respectively. In addition, strA and strB were detected in 78.1 and 65.6 % isolates resistant to streptomycin, respectively. Twenty-one isolates presented reduced susceptibility to β-lactams and harbored bla TEM, bla CMY, and/or bla CTX-M. Forty isolates showed reduced susceptibility to tetracycline, and most presented tet genes. These results highlight the importance of the environment as a reservoir of resistant Salmonella, which may enable the persistence of resistance determinants in the poultry production chain, contributing, therefore, to the debate regarding the impacts that antimicrobial use in animal production may exert in human health.

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Acknowledgments

The authors thank the Laboratório Porto Belo for supplying the majority of the Salmonella spp. used in this study. S. P. Mattiello received a scholarship from Probolsas/PUCRS.

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  1. Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Av. Ipiranga 6681, Porto Alegre, 90619-900, Brazil

    Samara P. Mattiello, Guilherme Drescher, Valdir C. Barth Jr., Carlos A. S. Ferreira & Sílvia D. Oliveira

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  1. Samara P. Mattiello
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  2. Guilherme Drescher
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  3. Valdir C. Barth Jr.
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Correspondence to Sílvia D. Oliveira.

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Mattiello, S.P., Drescher, G., Barth, V.C. et al. Characterization of antimicrobial resistance in Salmonella enterica strains isolated from Brazilian poultry production. Antonie van Leeuwenhoek 108, 1227–1238 (2015). https://doi.org/10.1007/s10482-015-0577-1

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