Abstract
A yellow-pigmented, Gram-stain negative and facultatively anaerobic bacterium, designated MF326T, was isolated from a sample of sediment collected from a sea cucumber culture pond in Rongcheng, China (122°14′34″E 36°54′36″N). Cells of strain MF326T were found to be catalase negative and oxidase positive. Optimal growth was found to occur at 30 °C and pH 7.0–7.5 in the presence of 2.0–3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MF326T is a member of the genus Mesonia and exhibits the high sequence similarity (94.3 %) with the type strain of Mesonia ostreae, followed by Mesonia algae (93.9 %). The dominant fatty acids of strain MF326T were identified as iso-C15:0, an unidentified fatty acid with an equivalent chain-length of 13.565 and anteiso-C15:0. The major polar lipids were found to be two unidentified lipids and phosphatidylethanolamine. The major respiratory quinone was found to be MK-6 and the genomic DNA G+C content was determined to be 40.7 mol%. On the basis of the phylogenetic analysis and differential phenotypic characteristics, it is concluded that strain MF326T (=KCTC 42255T =MCCC 1H00125T) should be assigned to the genus Mesonia as the type strain of a novel species, for which the name Mesonia sediminis sp. nov. is proposed.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (31370057, 31370108), National Science and Technology Major Project of China (2013ZX10004217) and 2013 Shandong Provincial Second Group Projects on Resource Platforms for Marine Economic and Innovative Development Regions: Marine Microorganisms Preservation Platform (2150299).
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The GenBank accession number for the 16S rRNA gene sequence of Mesonia sediminis MF326T is KR061432.
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Wang, FQ., Xie, ZH., Zhao, JX. et al. Mesonia sediminis sp. nov., isolated from a sea cucumber culture pond. Antonie van Leeuwenhoek 108, 1205–1212 (2015). https://doi.org/10.1007/s10482-015-0575-3
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DOI: https://doi.org/10.1007/s10482-015-0575-3