Abstract
A novel facultatively anaerobic and Gram-stain-negative bacterial strain, designated 5E3T, was isolated from intertidal sediments of Xiaoshi Island of Weihai, People’s Republic of China. Cells of strain 5E3T were long rod-shaped with widths of 0.3–0.5 μm and lengths of 4.0–6.0 μm. Optimum growth of strain 5E3T occurred at 33 °C, pH 6.5–7.0 and with 3% (w/v) NaCl. Oxidase activity was negative but catalase activity was weakly positive. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain 5E3T was most closely related to ‘Maribellus comscasis’ WC007 (99.5%), followed by M. sediminis MCCC 1K04285T (95.9%) and M. luteus XSD2T (95.6%). Genome comparisons between strain 5E3T and strain ‘M. comscasis’ WC007, using average nucleotide identity (ANI) value (93.0%) and DNA–DNA hybridization (DDH) value (50.1%), confirmed low genome relatedness. The major cellular fatty acids (≥ 10%) were iso-C15:0 and iso-C17:0 3–OH. The sole respiratory quinone was MK-7. The polar lipids consisted of phosphatidylethanolamine (PE), one unidentified aminolipid (AL) and three unidentified lipids (L1, L2, L3). The DNA G + C content was 37.9 mol%. According to the integrated results of phylogenetic, physiological, biochemical and chemotaxonomic characteristics, we propose that strain 5E3T represents a novel species of the genus Maribellus, for which the name Maribellus maritimus sp. nov. is proposed. The type strain is 5E3T (= KCTC 82744T = MCCC 1H00473T).
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References
Aziz RK, Bartels D, Best AA et al (2008) The RAST Server: rapid annotations using subsystems technology. BMC Genom 9:75. https://doi.org/10.1186/1471-2164-9-75
Bowman JP (2000) Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868. https://doi.org/10.1099/00207713-50-5-1861
Dong XZ, Cai MY (2001) Determination of biochemical characteristics. Manual for the systematic identification of general bacteria, 14th edn. Science Press, Beijing, pp 370–398
Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797. https://doi.org/10.1093/nar/gkh340
Goris J, Konstantinidis KT, Klappenbach JA et al (2007) DNA–DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57:81–91. https://doi.org/10.1099/ijs.0.64483-0
Hiraishi A, Ueda Y, Ishihara J, Mori T (1996) Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469
Huang ZB, Hu YZ, Lai QL, Guo Y (2020) Description of Maribellus sediminis sp. Nov., a marine nitrogen-fixing bacterium isolated from sediment of cordgrass and mangrove. Syst Appl Microbiol 43:126099. https://doi.org/10.1016/j.syapm.2020.126099
Huerta-Cepas J, Forslund K, Coelho LP et al (2017) Fast genome-wide functional annotation through orthology assignment by eggNOG-mapper. Mol Biol Evol 34:2115–2122. https://doi.org/10.1093/molbev/msx148
Jorgensen JH, Turnidge JD (2015) Susceptibility test methods: dilution and disk diffusion methods. In: Jorgensen JH, Carroll KC, Funke G et al (eds) Manual of clinical microbiology, 11th edn. Wiley, New York, pp 1253–1273. https://doi.org/10.1128/9781555817381.ch71
Kanehisa M, Sato Y, Kawashima M, Furumichi M, Tanabe M (2016) KEGG as a reference resource for gene and protein annotation. Nucleic Acids Res 44:457–462. https://doi.org/10.1093/nar/gkv1070
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549. https://doi.org/10.1093/molbev/msy096
Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, Chichester, pp 115–176
Li RQ, Yu C, Li YR et al (2009) SOAP2: an improved ultrafast tool for short read alignment. Bioinformatics 25:1966–1967. https://doi.org/10.1093/bioinformatics/btp336
Liang QY, Xu ZX, Zhang J, Chen GJ, Du ZJ (2018) Salegentibacter sediminis sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from coastal sediment. Int J Syst Evol Microbiol 68:2375–2380. https://doi.org/10.1099/ijsem.0.002849
Meier-Kolthoff JP, Auch AF, Klenk H, Göker M (2013) Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinform 14:60. https://doi.org/10.1186/1471-2105-14-60
Minnikin DE, O’Donnell AG, Goodfellow M et al (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Meth 2:233–241. https://doi.org/10.1016/0167-7012(84)90018-6
Mu DS, Liang QY, Wang XM et al (2018) Metatranscriptomic and comparative genomic insights into resuscitation mechanisms during enrichment culturing. Microbiome 6:230. https://doi.org/10.1186/s40168-018-0613-2
Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids MIDI technical note 101. MIDI Inc, Newark
Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray R, Wood W, Krieg N (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654
Yoon SH, Ha SM, Lim J, Kwon S, Chun J (2017) A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie Van Leeuwenhoek 110:1281–1286. https://doi.org/10.1007/s10482-017-0844-4
Zheng RK, Cai R, Liu R, Liu G, Sun CM (2021) Maribellus comscasis sp. nov., a novel deep-sea Bacteroidetes bacterium, possessing a prominent capability of degrading cellulose. Environ Microbiol 23:4561–4575. https://doi.org/10.1111/1462-2920.15650
Zhou LY, Yu ZL, Xu W, Mu DS, Du ZJ (2019) Maribellus luteus gen. nov., sp. Nov., a marine bacterium in the family Prolixibacteraceae isolated from coastal seawater. Int J Syst Evol Microbiol 69:2388. https://doi.org/10.1099/ijsem.0.003495
Zwickl D, Holder M (2004) Model parameterization, prior distributions, and the general time-reversible model in Bayesian phylogenetics. Syst Biol 53:877–888. https://doi.org/10.1080/10635150490522584
Acknowledgements
The implementation of scanning electron microscope was supported by the Physical-Chemical Materials Analytical and Testing Center of Shandong University at Weihai.
Funding
This work was supported by the National Natural Science Foundation of China (32070002, 31770002) and National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700).
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Ye, YQ., Han, ZT., Liu, XJ. et al. Maribellus maritimus sp. nov., isolated from marine sediment. Arch Microbiol 204, 40 (2022). https://doi.org/10.1007/s00203-021-02662-3
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DOI: https://doi.org/10.1007/s00203-021-02662-3