Paenibacillus populi sp. nov., a novel bacterium isolated from the rhizosphere of Populus alba

Abstract

A novel aerobic bacterium, designated strain LAM0705T, was isolated from the rhizosphere of Populus alba in the Peking University Third Hospital. Cells of strain LAM0705T were observed to be Gram-stain positive, motile, spore-forming and rod-shaped. The optimal temperature and pH for growth were found to be 30 °C and pH 7.5, respectively. Strain LAM0705T was found to be able to grow in the presence 0–5 % NaCl (w/v) (optimum 1.0 %). The major fatty acids of strain LAM0705T were identified as anteiso-C15:0, C16:0 and iso-C16:0. The dominant polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The cell wall peptidoglycan of strain LAM0705T was found to contain meso-diaminopimelic acid. The predominant menaquinone was identified as MK-7. The G+C content of genomic DNA was found to be 48 mol% when determined by the T m method. The 16S rRNA gene sequence similarity analysis indicated that strain LAM0705T is closely related to Paenibacillus agaridevorans DSM 1355T and Paenibacillus thailandensis KCTC 13043T with 97.8 and 96.1 % sequence similarity, respectively. The DNA–DNA hybridization value between strain LAM0705T and P. agaridevorans DSM 1355T was 47 ± 0.8 %. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM0705T is concluded to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus populi sp. nov. is proposed. The type strain is LAM0705T (=ACCC 06427T = JCM 19843T).

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Acknowledgments

This work was supported by National Nonprofit Institute Research Grant of CAAS (No. 2014-30), National Key Technology R&D Program of China (No. 2013BAD05B04F02 and 2011BAD11B05), Foundation of the Key Laboratory of Development and Application of Rural Renewable Energy (MOA, China) (No. 2013002), Science Foundation of Modern Farming Group (No. MF20100518), and Science Foundation of Dajing Group.

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Correspondence to Zhi-Yong Ruan.

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Tong-Yan Han and Xiao-Mei Tong contributed equally to the work and share first authorship.

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Han, TY., Tong, XM., Wang, YW. et al. Paenibacillus populi sp. nov., a novel bacterium isolated from the rhizosphere of Populus alba . Antonie van Leeuwenhoek 108, 659–666 (2015). https://doi.org/10.1007/s10482-015-0521-4

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Keywords

  • Paenibacillus populi sp. nov.
  • Polyphasic taxonomy
  • 16S rRNA gene
  • Rhizosphere
  • Populus alba