Pseudovibrio hongkongensis sp. nov., isolated from a marine flatworm


A Gram-negative, motile, rod-shaped bacterial strain, designated UST20140214-015BT, was isolated from a marine flatworm (Polyclad). The bacterium was found to be Gram-negative, oxidase and catalase positive, long-rod shaped, and motile by means of several peritrichous or subpolar flagella. Strain UST20140214-015BT grew optimally at 28 °C, at pH 7.0 and in the presence of 3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain UST20140214-015BT belongs to the genus Pseudovibrio, with highest sequence similarity to Pseudovibrio japonicus WSF2T (96.9 %), followed by P. ascidiaceicola F423T (96.7 %), P. denitrificans DN34T (96.6 %), P. axinellae Ad2T (96.3 %). All the other species shared <95.5 % sequence similarity. The principal fatty acids were identified as summed feature 8 (C18:1 ω7c/ω6c, as defined by the MIDI system; 70.8 %), C16:0 (9.1 %), summed feature 3 (C16:1 ω6c/C16:1 ω7c; 5.7 %). The G+C content of the chromosomal DNA was determined to be 53.3 mol%. The combined genotypic and phenotypic data show that strain UST20140214-015BT represents a novel species within the genus Pseudovibrio, for which the name Pseudovibrio hongkongensis sp. nov. is proposed, with the type strain UST20140214-015BT (=KCTC 42383T = MCCC 1K00451T).

This is a preview of subscription content, access via your institution.

Fig. 1



Marine Culture Collection of China


Korean Collection for Type Cultures


  1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman J, Smith JA, Struhl K (2002) Short protocols in molecular biology: a compendium of methods from current protocols in molecular biology. Wiley, Hoboken

    Google Scholar 

  2. Dong X, Cai M (2001) Determinative manual for routine bacteriology. Scientific Press (English translation), Beijing

    Google Scholar 

  3. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    CAS  PubMed  Article  Google Scholar 

  4. Fukunaga Y, Kurahashi M, Tanaka K, Yanagi K, Yokota A, Harayama S (2006) Pseudovibrio ascidiaceicola sp. nov., isolated from ascidians (sea squirts). Int J Syst Evol Microbiol 56:343–347

    CAS  PubMed  Article  Google Scholar 

  5. Hosoya S, Yokota A (2007) Pseudovibrio japonicas sp. nov., isolated from coastal seawater in Japan. Int J Syst Evol Microbiol 57:1952–1955

    CAS  PubMed  Article  Google Scholar 

  6. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    CAS  PubMed  Article  Google Scholar 

  7. Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acids techniques in bacterial systematics. Wiley, Chichester, pp 115–175

    Google Scholar 

  8. Mesbah M, Whitman WB (1989) Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr 479:297–306

    CAS  PubMed  Article  Google Scholar 

  9. O’Halloran JA, Barbosa TM, Morrissey JP, Kennedy J, Dobson AD, O’Gara F (2014) Pseudovibrio axinellae sp. nov., isolated from an Irish marine sponge. Int J Syst Envol Microbiol 63:141–145

    Article  Google Scholar 

  10. Rzhetsky A, Nei M (1992) Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 35:367–375

    CAS  PubMed  Article  Google Scholar 

  11. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  12. Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:16

    Google Scholar 

  13. Shieh WY, Lin Y-T, Jean WD (2004) Pseudovibrio denitrificans gen. nov., sp. nov., a marine, facultatively anaerobic, fermentative bacterium capable of denitrification. Int J Syst Evol Microbiol 54:2307–2312

    CAS  PubMed  Article  Google Scholar 

  14. Skerman V (1967) A guide to the identification of the genera of bacteria: with methods and digests of generic characteristics. Williams & Wilkins, Baltimore

    Google Scholar 

  15. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739

    CAS  PubMed Central  PubMed  Article  Google Scholar 

Download references


This work was financially supported by the National High Technology Research and Development Program of China (863 program, No. 2012AA092103) and Seed Funding from Scientific and Technical Innovation Council of Shenzhen Government (Grant No. 827000012).

Author information



Corresponding author

Correspondence to Yu Zhang.

Additional information

Ying Xu, Qi Li are contributed equally to this work.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 16 kb)

Supplementary material 2 (DOCX 157 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Xu, Y., Li, Q., Tian, R. et al. Pseudovibrio hongkongensis sp. nov., isolated from a marine flatworm. Antonie van Leeuwenhoek 108, 127–132 (2015).

Download citation


  • Pseudovibrio hongkongensis sp. nov.
  • Flatworm
  • Taxonomy