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Hymenobacter latericoloratus sp. nov. and Hymenobacter luteus sp. nov., isolated from freshwater sediment

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Abstract

Two novel Gram-stain negative, non-motile, rod-shaped and aerobic bacterial strains, designated YIM 77920T and YIM 77921T, were isolated from freshwater sediment of Jiuxiang cave, a tourism cave located in Yiliang county, Yunnan province, south-west China. The 16S rRNA gene sequences of strains YIM 77920T and YIM 77921T exhibited sequence similarities of 96.59 and 96.66 % to Hymenobacter xinjiangensis X2-YT, respectively, and indicated that the two isolates belong to the genus Hymenobacter. The major fatty acids present in the two strains were identified as C16:1 ω5c, iso-C15:0 and Summed Feature 4 (C17:1 anteiso B/iso I). MK-7 was identified as the respiratory quinone component for both strains. The polar lipids profile of strain YIM 77920T was found to consist of phosphatidylethanolamine, four unidentified polar lipids, three unidentified aminophospholipids, two unidentified phospholipids and two unidentified aminolipids, while that of strain YIM 77921T consisted of phosphatidylethanolamine, four unidentified polar lipids, two unidentified aminolipids, one unidentified phospholipid and four unidentified aminophospholipids. The DNA G+C contents of strains YIM 77920T and YIM 77921T were determined to be 57.5 and 59.6 mol%, respectively. DNA–DNA hybridization between them had a low value (56.55 %). Based on the morphological and physiological properties, and phylogenetic analyses, strains YIM 77920T and YIM 77921T are considered to represent two novel species of the genus Hymenobacter, for which the names Hymenobacter latericoloratus sp. nov. (type strain YIM 77920T = JCM 30327T = CCTCC AB 2012949T) and Hymenobacter luteus sp. nov. (type strain YIM 77921T = JCM 30328T = CCTCC AB 2012947T) are proposed.

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Acknowledgments

We are grateful to Prof. Dr. Takuji Kudo (JCM) for his kind providing reference type strain and Prof. Aharon Oren (The Hebrew University of Jerusalem, Israel) for his kind help with the Latin etymology for the new species. This work was supported by the National Natural Science Foundation of China (No. 81102806), the Deanship of Scientific Research at King Saud University for funding this work through the research group no RGP-205, the opening project of the State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences (No. SKLMR-20100602), Yunnan Provincial Natural Science Foundation (2011FZ166, 2013FA004). W-J Li was also supported by Guangdong Province Higher Vocational Colleges & Schools Pearl River Scholar Funded Scheme (2014).

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Authors and Affiliations

  1. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Lan Liu, En-Min Zhou, Jian-Yu Jiao, Deene Manikprabhu, Hong Ming & Wen-Jun Li

  2. State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, 510275, People’s Republic of China

    En-Min Zhou, Deene Manikprabhu, Wen-Sheng Shu & Wen-Jun Li

  3. Medical Faculty of Kunming University of Science and Technology, Kunming University of Science and Technology, Kunming, 650500, People’s Republic Of China

    Jian-Yu Jiao

  4. Department of Agriculture and Biological Sciences, Dali University, Dali, 671003, People’s Republic of China

    Wei-Hong Liu

  5. Bioproducts Research Chair (BRC), College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

    Wael N. Hozzein

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  1. Lan Liu
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  2. En-Min Zhou
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Correspondence to Wen-Jun Li.

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Lan Liu and En-Min Zhou have contributed equally to this work.

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Liu, L., Zhou, EM., Jiao, JY. et al. Hymenobacter latericoloratus sp. nov. and Hymenobacter luteus sp. nov., isolated from freshwater sediment. Antonie van Leeuwenhoek 107, 165–172 (2015). https://doi.org/10.1007/s10482-014-0314-1

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