Abstract
A Gram-stain negative, non-flagellated, non-gliding and rod-shaped bacterium, strain JHH-2T, was isolated from seawater collected in Jeju Island, Korea. The novel isolate was found to grow at 25–30 °C, at pH 6.5–7.0 and in the presence of 1–2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JHH-2T is closely related to Siansivirga zeaxanthinifaciens JCM 17682T, with 16S rRNA gene sequence similarity of 96.6 %. The predominant respiratory quinone was identified as menaquinone-6 and the major cellular fatty acids were detected as iso-C15:0, C16:0 and iso-C17:0 3-OH. Polar lipid profiling revealed the presence of phosphatidylethanolamine, an unidentified glycolipid, four unidentified aminolipids and two unidentified lipids. The DNA G+C content of the chromosomal DNA of the type strain is 35.6 mol%. On the basis of several distinct phenotypic characteristics and phylogenetic analysis, a new species of the genus Siansivirga, Siansivirga jejunensis JHH-2T sp. nov. is proposed. The type strain is JHH-2T (= KCCM 92030T = JCM 19228T).
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References
Bernardet JF, Nakagawa Y, Holmes B and Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes (2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J SystEvol Microbiol 52:1049–1070
Bowman JP (2000) Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868
Breznak JA, Costilow RN (1994) Physicochemical factors in growth. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 137–154
Hall TA (1999) Bioedit: a user-friendly biological sequence alignment editor and analysis135 program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Hameed A, Shahina M, Lin SY, Sridhar KR, Young LS, Lee MR, Chen WM, Chou JH, Young CC (2012) Siansivirga zeaxanthinifaciens gen. nov., sp. nov., a novel zeaxanthin-producing member of the family Flavobacteriaceae isolated from coastal seawater of Taiwan. FEMS Microbiol Lett 333:37–45
Jorgensen JH, Turnidge JD, Washington JA (1999) Antibacterial susceptibility tests: dilution and disk diffusion methods. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH (eds) Manual of clinical microbiology. American Society for Microbiology, Washington, DC, pp 1526–1543
Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA Gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721
Komagata K, Suzuki K (1987) Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
Lee DG, Park JM, Kang H, Hong SY, Lee KR, Chang HB, Trujillo ME (2013) Asinibacterium lactis gen. nov., sp. nov., a member of the family Chitinophagaceae, isolated from donkey (Equus asinus) milk. Int J Syst Evol Microbiol 63:3180–3185
Minnikin DE, O’onnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241
Park SC, Choe HN, Baik KS, Lee KH, Seong CN (2012) Gaetbulibacter aestuarii sp. nov., isolated from shallow coastal seawater, and emended description of the genus Gaetbulibacter. Int J Syst Evol Microbiol 62:150–154
Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI Inc, Newark
Seldin L, Dubnau D (1985) Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35:151–154
Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed phase high performance liquid chromatography. FEMS Microbiol Lett 25:125–128
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
Yang SJ, Cho JC (2008) Gaetbulibacter marinus sp. nov., isolated from coastal seawater, and emended description of the genus Gaetbulibacter. Int J Syst Evol Microbiol 58:315–318
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This work was supported by project National Coordinating Center for Global Cosmetics R&D (A103017) of the Foundation of Korea Cosmetic Industry Institute, Republic of Korea.
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Park, JM., Han, SK., Lee, DG. et al. Siansivirga jejunensis sp. nov., isolated from seawater of Jeju Island in Korea and emendation of the genus Siansivirga . Antonie van Leeuwenhoek 106, 763–769 (2014). https://doi.org/10.1007/s10482-014-0246-9
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DOI: https://doi.org/10.1007/s10482-014-0246-9