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Micromonospora taraxaci sp. nov., a novel endophytic actinomycete isolated from dandelion root (Taraxacum mongolicum Hand.-Mazz.)

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Abstract

A novel actinomycete, designated strain NEAU-P5T, was isolated from dandelion root (Taraxacum mongolicum Hand.-Mazz.). Strain NEAU-P5T showed closest 16S rRNA gene sequence similarity to Micromonospora chokoriensis 2–19/6T (99.5 %), and phylogenetically clustered with Micromonospora violae NEAU-zh8T (99.3 %), M. saelicesensis Lupac 09T (99.0 %), M. lupini Lupac 14NT (98.8 %), M. zeae NEAU-gq9T (98.4 %), M. jinlongensis NEAU-GRX11T (98.3 %) and M. zamorensis CR38T (97.9 %). Phylogenetic analysis based on the gyrB gene sequence also indicated that the isolate clustered with the above type strains except M. violae NEAU-zh8T. The cell-wall peptidoglycan consisted of meso-diaminopimelic acid and glycine. The major menaquinones were MK-9(H8), MK-9(H6) and MK-10(H2). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were C16:0, iso-C15:0 and C17:0. Furthermore, some physiological and biochemical properties and low DNA–DNA relatedness values enabled the strain to be differentiated from members of closely related species. Therefore, it is proposed that strain NEAU-P5T represents a novel species of the genus Micromonospora, for which the name Micromonospora taraxaci sp. nov. is proposed. The type strain is NEAU-P5T (=CGMCC 4.7098T = DSM 45885T).

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Acknowledgments

This work was supported in part by grants from the National Outstanding Youth Foundation (No. 31225024), the National Key Project for Basic Research (No. 2010CB126102), the National Key Technology R&D Program (No. 2012BAD19B06), the Program for New Century Excellent Talents in University (NCET-11-0953), the National Natural Science Foundation of China (No.31372006, 31171913 and 31071750), the Outstanding Youth Foundation of Heilongjiang Province (JC201201), the Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP), the Sicence and Technology Research Project of Heilongjiang Provincial Educational Commission (No. 12541001) and the Youth Science Foundation of Heilongjiang Province (No. QC2014C013).

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Authors and Affiliations

  1. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Junwei Zhao, Lifeng Guo, Hairong He, Chongxi Liu, Yuejing Zhang, Chuang Li, Xiangjing Wang & Wensheng Xiang

  2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

    Wensheng Xiang

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  1. Junwei Zhao
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  2. Lifeng Guo
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  3. Hairong He
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  5. Yuejing Zhang
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  7. Xiangjing Wang
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Correspondence to Xiangjing Wang or Wensheng Xiang.

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Junwei Zhao and Lifeng Guo have contributed equally to this work.

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Zhao, J., Guo, L., He, H. et al. Micromonospora taraxaci sp. nov., a novel endophytic actinomycete isolated from dandelion root (Taraxacum mongolicum Hand.-Mazz.). Antonie van Leeuwenhoek 106, 667–674 (2014). https://doi.org/10.1007/s10482-014-0237-x

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