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Micromonospora maoerensis sp. nov., isolated from a Chinese pine forest soil

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Abstract

A novel actinomycete, designated strain NEAU-MES19T, was isolated from pine forest soil in Heilongjiang province, China. A polyphasic study was carried out to establish the taxonomic position of this strain. The organism was found to have morphological and chemotaxonomic characteristics typical of the genus Micromonospora. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain NEAU-MES19T was most closely related to Micromonospora matsumotoense IMSNU 22003T. However, phylogenetic analysis based on the gyrB gene sequence showed that the isolate was more closely related to Micromonospora cremea CR30T than M. matsumotoense IMSNU 22003T. The low level of DNA–DNA relatedness allowed the isolate to be differentiated from M. matsumotoense IMSNU 22003T and M. cremea CR30T. Moreover, strain NEAU-MES19T could also be distinguished from its closest phylogenetic relatives by morphological, physiological and biochemical characteristics. Therefore, it is proposed that strain NEAU-MES19T represents a novel species of the genus Micromonospora, for which the name Micromonospora maoerensis sp. nov. is proposed. The type strain is NEAU-MES19T (=CGMCC 4.7091T = DSM 45884T).

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Acknowledgments

This work was supported in part by grants from the National Outstanding Youth Foundation (No. 31225024), the National Key Project for Basic Research (No. 2010CB126102) the National Key Technology R&D Program (No. 2012BAD19B06), the Program for New Century Excellent talents in University (NCET-11-0953), the National Natural Science Foundation of China (Nos .31372006, 31171913 and 31071750), the Outstanding Youth Foundation of Heilongjiang Province (JC201201) and Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP).

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Authors and Affiliations

  1. School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Chuang Li, Chongxi Liu, Junwei Zhao, Yuejing Zhang, Ruixia Gao, Xinhui Zhang & Wensheng Xiang

  2. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Harbin, 150030, People’s Republic of China

    Meng Yao & Xiangjing Wang

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  1. Chuang Li
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Correspondence to Xiangjing Wang or Wensheng Xiang.

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Chuang Li and Chongxi Liu have contributed equally to this work.

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Li, C., Liu, C., Zhao, J. et al. Micromonospora maoerensis sp. nov., isolated from a Chinese pine forest soil. Antonie van Leeuwenhoek 105, 451–459 (2014). https://doi.org/10.1007/s10482-013-0096-x

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