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Motiliproteus sediminis gen. nov., sp. nov., isolated from coastal sediment

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Abstract

A novel Gram-stain-negative, rod-to-spiral-shaped, oxidase- and catalase- positive and facultatively aerobic bacterium, designated HS6T, was isolated from marine sediment of Yellow Sea, China. It can reduce nitrate to nitrite and grow well in marine broth 2216 (MB, Hope Biol-Technology Co., Ltd) with an optimal temperature for growth of 30–33 °C (range 12–45 °C) and in the presence of 2–3 % (w/v) NaCl (range 0.5–7 %, w/v). The pH range for growth was pH 6.2–9.0, with an optimum at 6.5–7.0. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that the novel isolate was 93.3 % similar to the type strain of Neptunomonas antarctica, 93.2 % to Neptunomonas japonicum and 93.1 % to Marinobacterium rhizophilum, the closest cultivated relatives. The polar lipid profile of the novel strain consisted of phosphatidylethanolamine, phosphatidylglycerol and some other unknown lipids. Major cellular fatty acids were summed feature 3 (C16:1 ω7c/iso-C15:0 2-OH), C18:1 ω7c and C16:0 and the main respiratory quinone was Q-8. The DNA G+C content of strain HS6T was 61.2 mol %. Based on the phylogenetic, physiological and biochemical characteristics, strain HS6T represents a novel genus and species and the name Motiliproteus sediminis gen. nov., sp. nov., is proposed. The type strain is HS6T (=ATCC BAA-2613T=CICC 10858T).

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31370057, 31290231, 31370108) and the China Ocean Mineral Resources R & D Association (COMRA) Special Foundation (DY125-15-T-05). Z. J. Wang acknowledges grant of Graduate Innovation Foundation of Shandong University at WeiHai, GIFSDUWH yjs12037.

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Authors and Affiliations

  1. College of Marine Science, Shandong University at Weihai, Weihai, 264209, China

    Zong-Jie Wang, Chao Wang, Zong-Jun Du & Guan-Jun Chen

  2. Key Laboratory of Coastal Biology and Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China

    Zhi-Hong Xie

  3. State key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China

    Zong-Jun Du & Guan-Jun Chen

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  1. Zong-Jie Wang
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  2. Zhi-Hong Xie
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  3. Chao Wang
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  4. Zong-Jun Du
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  5. Guan-Jun Chen
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Corresponding author

Correspondence to Zong-Jun Du.

Additional information

The GenBank accession number for the 16S rRNA gene sequence of Motiliproteus sediminis HS6T is KF953945.

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Wang, ZJ., Xie, ZH., Wang, C. et al. Motiliproteus sediminis gen. nov., sp. nov., isolated from coastal sediment. Antonie van Leeuwenhoek 106, 615–621 (2014). https://doi.org/10.1007/s10482-014-0232-2

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  • DOI: https://doi.org/10.1007/s10482-014-0232-2

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