Abstract
A Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strain, designated NY5T, was isolated from marine sediment collected from coastal area in Weihai, China (122°07′ 38.80′′ E, 37°33′ 57.60′′ N). Cells of strain NY5T were 0.6–0.7 μm width and 1.9–2.0 μm length, catalase-positive and oxidase-positive. Growth of NY5T was observed at 25–37 °C (optimum, 28 °C) and pH 6.5–9.5 (optimum, pH 7.5–8.0) and in the presence of 0.5–7.0% (w/v) NaCl (optimum, 2.0%). The isoprenoid quinone was Q-8 and the predominant fatty acids were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and C17:1 ω8c. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids. The DNA G+C content of strain NY5T was 60.1%. Strain NY5T showed the highest 16S rRNA gene sequence similarity (98.2%) with Pseudohalioglobus lutimaris followed by Parahaliea aestuarii (96.9%), Parahaliea maris (96.7%), Parahaliea mediterranea (95.9%), and Halioglobus japonicus (94.9%). Given these phenotypic and chemotaxonomic properties and phylogenetic analyses, strain NY5T was considered to represent a novel species of the genus Pseudohalioglobus, for which the name Pseudohalioglobus sediminis sp. nov. is proposed. The type strain is NY5T (=KCTC 72416T=MCCC 1H00401T).
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The GenBank accession number for the 16S rRNA gene sequence of strain NY5T is MT705158. The whole-genome shotgun project of strain NY5T has been deposited at DDBJ/ENA/GenBank under the accession number VTUX00000000.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (32070002, 31900091, 31770002), the National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700), the China Postdoctoral Science Foundation (2019M652369) and the State Key Laboratory of Microbial Technology Open Projects Fund (M2019-08). This work of scanning electron microscope was supported by the Physical-Chemical Materials Analytical & Testing Center of Shandong University at Weihai.
Funding
This work was supported by the National Natural Science Foundation of China (32070002, 31900091, 31770002), the National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700), the China Postdoctoral Science Foundation (2019M652369) and the State Key Laboratory of Microbial Technology Open Projects Fund (M2019-08).
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Strain NY5T was isolated by Y-NZ and T-SZ. Material preparation, data collection and analyses were performed by Y-NZ, T-SZ, X-YC, YG and Z-JD. The manuscript was written by Y-NZ, T-SZ, YG and Z-JD. Project guidance and critical revision of manuscripts were performed by YG and Z-JD. All authors read and approved the final manuscript.
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Zhang, YN., Zhang, TS., Chen, XY. et al. Pseudohalioglobus sediminis sp. nov., isolated from coastal sediment. Arch Microbiol 204, 207 (2022). https://doi.org/10.1007/s00203-022-02816-x
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DOI: https://doi.org/10.1007/s00203-022-02816-x