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Nocardia casuarinae sp. nov., an actinobacterial endophyte isolated from root nodules of Casuarina glauca

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Abstract

An actinobacterium strain BMG51109a was isolated from surface sterilized root nodules of Casuarina glauca collected in Tunisia. The 16S rRNA gene sequence of strain BMG51109a showed most similarity (96.53–96.55 %) to the type strains of Nocardia transvalensis, N. aobensis and N. elegans. Chemotaxonomic analysis supported the assignment of the strain to Nocardia genus. The major menaquinone was MK-8(H4c) while the polar lipid profile contained diphosphatidylglycerol, phosphatidylmonomethylethanolamine, glycophospholipid, phosphatidylinositol, one uncharacterized phospholipid and three glycolipids. Whole-cell sugar analysis revealed the presence of meso-diaminopimelic acid, arabinose and galactose as diagnostic sugars, complemented by glucose, mannose and ribose. The major cellular fatty acids were tuberculostearic, oleic, palmitoleic and stearic acids. Physiological and biochemical tests showed that strain BMG51109a could be clearly distinguished from its closest phylogenetic neighbours. On the basis of these results, strain BMG51109aT (= DSM 45978T = CECT 8469T) is proposed as the type strain of the novel species Nocardia casuarinae sp. nov.

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Acknowledgments

The authors would like to gratefully acknowledge Prof. Manfred Rohde (Helmholz Centre for Infection Biology, Braunschweig, Germany) for providing the Scanning EM Photo in Fig. 2b. This research was supported by The Ministère de l’Enseignement Supérieur et de la Recherche Scientifique, Tunisie.

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Authors and Affiliations

  1. Laboratoire Microorganismes et Biomolécules Actives, Université Tunis El Manar (FST) & Université Carthage (INSAT), Campus Universitaire, 2092, Tunis, Tunisia

    Faten Ghodhbane-Gtari, Imen Nouioui, Karima Salem, Amir Ktari & Maher Gtari

  2. Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Brunswick, Germany

    Maria del Carmen Montero-Calasanz & Hans-Peter Klenk

  3. Department of Molecular, Cellular, & Biomedical Sciences, University of New Hampshire, Durham, NH, USA

    Louis S. Tisa

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  1. Faten Ghodhbane-Gtari
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  2. Imen Nouioui
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  3. Karima Salem
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  4. Amir Ktari
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  8. Maher Gtari
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Correspondence to Maher Gtari.

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Ghodhbane-Gtari, F., Nouioui, I., Salem, K. et al. Nocardia casuarinae sp. nov., an actinobacterial endophyte isolated from root nodules of Casuarina glauca . Antonie van Leeuwenhoek 105, 1099–1106 (2014). https://doi.org/10.1007/s10482-014-0168-6

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