Abstract
In total, 435 pure bacterial strains were isolated from microtherm oil-production water from the Karamay Oilfield, Xinjiang, China, by using four media: oil-production water medium (Cai medium), oil-production water supplemented with mineral salt medium (CW medium), oil-production water supplemented with yeast extract medium (CY medium), and blood agar medium (X medium). The bacterial isolates were affiliated with 61 phylogenetic groups that belong to 32 genera in the phyla Actinobacteria, Firmicutes, and Proteobacteria. Except for the Rhizobium, Dietzia, and Pseudomonas strains that were isolated using all the four media, using different media led to the isolation of bacteria with different functions. Similarly, nonheme diiron alkane monooxygenase genes (alkB/alkM) also clustered according to the isolation medium. Among the bacterial strains, more than 24 % of the isolates could use n-hexadecane as the sole carbon source for growth. For the first time, the alkane-degrading ability and alkB/alkM were detected in Rhizobium, Rhodobacter, Trichococcus, Micrococcus, Enterococcus, and Bavariicoccus strains, and the alkM gene was detected in Firmicutes strains.
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Acknowledgments
This research was supported by the National Natural Science Foundation of China (31200100, 31070107, and 31225001) and National High Technology Research and Development Program of China (2009AA063501 and 2013AA064401).
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Ji-Quan Sun and Lian Xu have contributed equally to this work.
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Sun, JQ., Xu, L., Zhang, Z. et al. Diverse bacteria isolated from microtherm oil-production water. Antonie van Leeuwenhoek 105, 401–411 (2014). https://doi.org/10.1007/s10482-013-0088-x
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DOI: https://doi.org/10.1007/s10482-013-0088-x