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Nonomuraea guangzhouensis sp. nov., and Nonomuraea harbinensis sp. nov., two novel actinomycetes isolated from soil

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Abstract

Two novel actinomycetes, designated strains NEAU-ZJ3T and NEAU-yn31T, were isolated from soils in China and their taxonomic positions determined by a polyphasic approach. Key morphological and chemotaxonomic properties of the two strains were consistent with the description of the genus Nonomuraea. The G+C contents of strains NEAU-ZJ3T and NEAU-yn31T were determined to be 67.4 and 68.0 %, respectively. Phylogenetic analysis using 16S rRNA gene sequences indicated that the two strains should be classified in the genus Nonomuraea. The 16S rRNA gene sequence of strain NEAU-ZJ3T showed it to be most closely related to Nonomuraea salmonea DSM 43678T (98.27 %), Nonomuraea endophytica YIM 65601T (98.23 %) and Nonomuraea candida HMC10T (98.08 %), and that of strain NEAU-yn31T to Nonomuraea ferruginea IFO 14094T (99.08 %) and N. candida HMC10T (98.01 %). Furthermore, a combination of DNA–DNA hybridization results and some phenotypic characteristics indicated that the two strains can be distinguished from each other and their phylogenetically closest relatives. Thus, strains NEAU-ZJ3T and NEAU-yn31T represent two novel species of the genus Nonomuraea, for which the names Nonomuraea guangzhouensis sp. nov. and Nonomuraea harbinensis sp. nov. are proposed. The type strains are NEAU-ZJ3T (=CGMCC 4.7101T = DSM 45889T) and NEAU-yn31T (=CGMCC 4.7106T = DSM 45887T), respectively.

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Acknowledgments

This work was supported in part by grants from the Opening Fund of Key Laboratory of Soybean Biology in Chinese Ministry of Education (No. SB12B04), the National Key Project for Basic Research (No. 2010CB126102), the National Outstanding Youth Foundation (No. 31225024), the Special Foundation for Scientific and Technological Innovation Research of Harbin (No. 2011RFXXN038) and the Natural Science Foundation of Heilongjiang Province (No. C201029).

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Authors and Affiliations

  1. Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang, Harbin, 150030, People’s Republic of China

    Shurui Wang & Chongxi Liu

  2. School of Life Science, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang, Harbin, 150030, People’s Republic of China

    Shurui Wang, Chongxi Liu, Yuejing Zhang, Junwei Zhao, Xinhui Zhang & Wensheng Xiang

  3. Key Laboratory of Microbial Drug Engineering, Heilongjiang Provincial Education Committee, Harbin, 150030, People’s Republic of China

    Lingyu Yang & Xiangjing Wang

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  1. Shurui Wang
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  2. Chongxi Liu
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  3. Yuejing Zhang
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  4. Junwei Zhao
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  5. Xinhui Zhang
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Correspondence to Xiangjing Wang or Wensheng Xiang.

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Shurui Wang and Chongxi Liu contributed equally to this work.

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Wang, S., Liu, C., Zhang, Y. et al. Nonomuraea guangzhouensis sp. nov., and Nonomuraea harbinensis sp. nov., two novel actinomycetes isolated from soil. Antonie van Leeuwenhoek 105, 109–118 (2014). https://doi.org/10.1007/s10482-013-0058-3

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