Abstract
Four yeast strains were isolated from rotting wood samples collected from two sites in the Baotianman Nature Reserve and the Laojieling Nature Reserve in China. DNA sequence comparison and other taxonomic characteristics identified the strains as a single novel species of the genus Metschnikowia. The name Metschnikowia henanensis sp. nov. is proposed to accommodate these highly divergent organisms with the type strain BY-97T (= CICC 1982T = CBS 12677T). The novel species produced chlamydospores, but it did not exhibit ascospore formation in sporulation media for 4 weeks. Molecular phylogeny from the D1/D2 domains of the large subunit (LSU) rRNA gene sequences placed this new species in a basal position to the Metschnikowia viticola/Candida kofuensis/Metschnikowia noctiluminum subclade, and an undescribed Candida species namely strains IMB-EMP4 and IMB-EMP5 was a close sister to M. henanensis.
Similar content being viewed by others
References
Barbosa AC, Cadete RM, Gomes FC, Lachance MA, Rosa CA (2009) Candida materiae sp. nov., a yeast species isolated from rotting wood in the Atlantic Rain Forest. Int J Syst Evol Microbiol 8:2104–2106
Cadete RM, Santos RO, Melo MA, Mouro A, Gonçalves DL, Stambuk BU, Gomes FC, Lachance MA, Rosa CA (2009) Spathaspora arborariae sp. nov., a d-xylose-fermenting yeast species isolated from rotting wood in Brazil. FEMS Yeast Res 9:1338–1342
Cadete RM, Melo MA, Lopes MR, Pereira GM, Zilli JE, Vital MJ, Gomes FC, Lachance MA, Rosa CA (2012) Candida amazonensis sp. nov., an ascomycetous yeast isolated from rotting wood in the Amazonian forest. Int J Syst Evol Microbiol 62:1438–1440
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
Grinbergs J, Yarrow D (1970) Two new Candida species: Candida chilensis sp.n. and Candida valdiviana sp.n. Antonie Van Leeuwenhoek 36:143–148
Guo X, Zhu H, Bai FY (2012) Candida cellulosicola sp. nov., a xylose-utilizing anamorphic yeast from rotten wood. Int J Syst Evol Microbiol 62:242–245
James SA, Carvajal Barriga EJ, Bond CJ, Cross K, Núñez NC, Portero PB, Roberts IN (2009) Candida carvajalis sp. nov., an ascomycetous yeast species from the Ecuadorian Amazon jungle. FEMS Yeast Res 9:784–788
Kimura M (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
Kurtzman CP (2006) Yeast species recognition from gene sequence analyses and other molecular methods. Mycoscience 47:65–71
Kurtzman CP, Robnett CJ (1998) Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 73:331–371
Lachance MA (2011) Metschnikowia Kamienski (1899). In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts, a taxonomic study, 5th edn. Elsevier, Amsterdam, pp 575–620
Lachance MA, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP (2011) Candida Berkhout (1923). In: Kurtzman CP, Fell JW, Boekhout T (eds) The yeasts, a taxonomic study, 5th edn. Elsevier, Amsterdam, pp 987–1279
Middelhoven WJ (2006) Polysaccharides and phenolic compounds as substrate for yeasts isolated from rotten wood and description of Cryptococcus fagi sp.nov. Antonie Van Leeuwenhoek 90:57–67
Middelhoven WJ, Kurtzman CP (2007) Four novel yeasts species from decaying organic matter: Blastobotrys robertii sp. nov., Candida cretensis sp. nov., Candida scorzettiae sp. nov. and Candida vadensis sp. nov. Antonie Van Leeuwenhoek 92:233–244
Péter G, Dlauchy D, Tornai-Lehoczki J, Gouliamova D, Kurtzman CP (2011a) Ogataea saltuana sp. nov., a novel methanol-assimilating yeast species. Antonie Van Leeuwenhoek 100:375–383
Péter G, Dlauchy D, Tornai-Lehoczki J, Suzuki M, Kurtzman CP (2011b) Spencermartinsiella europaea gen. nov., sp. nov., a new member of the family Trichomonascaceae. Int J Syst Evol Microbiol 61:993–1000
Ramírez C, González A (1984a) Two new species and one variety of nitrate-utilizing mycelial Candida isolated from decayed wood in the evergreen rainy Valdivian forest of southern Chile. Mycopathologia 88:55–60
Ramírez C, González A (1984b) Five new filamentous, glucose-fermenting Candida isolated from decayed wood in the evergreen rainy Valdivian forest of southern Chile. Mycopathologia 88:83–92
Ramos JP, Valente P, De Souza RA, Rosa CA, Leoncini O (2001) Heteroduplex mobility assay of the D1/D2 region of the 26S rDNA for differentiation of Saccharomyces species. Lett Appl Microbiol 33:206–210
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Santos RO, Cadete RM, Badotti F, Mouro A, Wallheim DO, Gomes FC, Stambuk BU, Lachance MA, Rosa CA (2011) Candida queiroziae sp. nov., a cellobiose-fermenting yeast species isolated from rotting wood in Atlantic Rain Forest. Antonie Van Leeuwenhoek 99:635–642
Scorzetti G, Fell JW, Fonseca A, Statzell-Tallman A (2002) Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res 2:495–517
Thanh VN, Hai DA, Lachance MA (2003) Issatchenkia hanoiensis, a new yeast species isolated from frass of the litchi fruit borer Conopomorpha cramerella Snellen. FEMS Yeast Res 4(1):113–117
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DJ (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882
Urbina H, Blackwell M (2012) Multilocus phylogenetic study of the Scheffersomyces yeast clade and characterization of the N-terminal region of xylose reductase gene. PLoS ONE 7:1–13
Wang C, Wang QM, Jia JH, Bai FY (2009) Candida pseudocylindracea sp. nov. and Candida wuzhishanensis sp. nov. from Hainan Island, southern China. Mycosystema 28:79–85
Wang SA, Li FL, Bai FY (2010) Candida laoshanensis sp. nov. and Candida qingdaonensis sp. nov., novel anamorphic, ascomycetous yeast species isolated from decayed wood. Int J Syst Evol Microbiol 60:1697–1701
Yamada Y, Kondo K (1973) Coenzyme Q system in the classification of the yeast genera Rhodotorula and Cryptococcus and the yeast-like genera Sporobolomyces and Rhodosporidium. J Gen Appl Microbiol 19:59–77
Yarrow D (1998) Methods for the isolation and identification of yeasts. In: Kurtzman CP, Fell JW (eds) The Yeasts, a taxonomic study, 4th edn. Elsevier, Amsterdam, pp 77–100
Acknowledgments
This work was supported by grant no.122300410032 from the Research Planning Project of Basic and Advanced Technology of Henan Province, China.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hui, FL., Chen, L., Li, ZH. et al. Metschnikowia henanensis sp. nov., a new anamorphic yeast species isolated from rotten wood in China. Antonie van Leeuwenhoek 103, 899–904 (2013). https://doi.org/10.1007/s10482-012-9871-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10482-012-9871-3