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Virgibacillus albus sp. nov., a novel moderately halophilic bacterium isolated from Lop Nur salt lake in Xinjiang province, China

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Abstract

A Gram-positive, moderately halophilic, strictly aerobic bacterium, designated YIM 93624T, was isolated from a salt lake in Xinjiang province of China and subjected to a polyphasic taxonomic study. Strain YIM 93624T grew at 15–45 °C (optimum 25–30 °C), 1–17% (w/v) NaCl (optimum 5–10 %, w/v) and pH 4.0–9.0 (optimum pH 7.0). The predominant menaquinone was found to be MK-7. The major fatty acids were anteiso-C15:0 and C16:0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, a glycolipid and two unidentified phospholipids. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The G+C content of the genomic DNA was 37.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 93624T was a member of the genus Virgibacillus and exhibited the highest similarity of 97.0 % to Virgibacillus koreensis KCTC 3823T. However, the level of DNA–DNA relatedness between strain YIM 93624T and V. koreensis KCTC 3823T was 32.5 %. On the basis of phylogenetic, physiological and chemotaxonomic analysis data, the isolate is concluded to represent a novel species of the genus Virgibacillus, for which the name Virgibacillus albus sp. nov., is proposed, with type strain of YIM 93624T (=DSM 23711T = JCM 17364T).

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Acknowledgments

The authors are grateful to Dr. Jung-Sook Lee from Korean Collection for Type Cultures (KCTC) for her kind providing reference type strain Virgibacillus koreensis KCTC 3823T. This work was supported by the National Natural Science Foundation of China (31000001, 30860002). W-J. Li was also supported by ‘Hundred Talents Program’ of the Chinese Academy of Sciences.

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Authors and Affiliations

  1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-Resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, China

    Yun-Jiao Zhang, Yu Zhou, Man Ja, Rong Shi, Wei-Xun Chun-Yu, Ling-Ling Yang, Shu-Kun Tang & Wen-Jun Li

  2. Zhejiang Province Key Laboratory for Food Safety, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, No. 198, Shiqiao Road, Hangzhou, 310021, Zhejiang, China

    Yu Zhou

  3. Key Laboratory of Biogeography and Bioresource in Arid Land, Chinese Academy of Science, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi, 830011, China

    Wen-Jun Li

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  1. Yun-Jiao Zhang
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  2. Yu Zhou
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  3. Man Ja
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Corresponding authors

Correspondence to Shu-Kun Tang or Wen-Jun Li.

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Yun-Jiao Zhang and Yu Zhou contributed equally to this study.

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Zhang, YJ., Zhou, Y., Ja, M. et al. Virgibacillus albus sp. nov., a novel moderately halophilic bacterium isolated from Lop Nur salt lake in Xinjiang province, China. Antonie van Leeuwenhoek 102, 553–560 (2012). https://doi.org/10.1007/s10482-012-9750-y

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