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The β-tubulin gene as a molecular phylogenetic marker for classification and discrimination of the Saccharomyces sensu stricto complex

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Abstract

The Saccharomyces sensu stricto complex comprises seven very closely related species. In this study, we compared the use of two different phylogenetic markers, the 26S rDNA and β-tubulin genes, for discriminating phylogenetic relationships among Saccharomyces sensu stricto strains using sequencing as well as RFLP methods. The average sequence similarity for the β-tubulin gene (90.0%) among seven strains was significantly less than that for 26S rDNA (98.6%). This result demonstrates that β-tubulin gene sequences provided higher resolution than 26S rDNA sequences. Species-specific restriction profiles of the Saccharomyces strains were obtained by cutting them with the Tsp509I enzyme. Our data indicate that phylogenetic relationships between these strains are best resolved using sequencing or RFLP analysis of the β-tubulin gene.

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Acknowledgments

We thank T. Y. Liu, C. C. Liao, G. F. Yuan (Food Industry Research and Development Institute, Taiwan), M. T. Chang (Department of Animal Science, National Chung Hsing University, Taiwan) for their encouragement. This research was supported by the Taiwanese Ministry of Economic Affairs (project no. 97-EC-17-A-R7-0525).

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  1. Bioresource Collection and Research Center, Food Industry Research and Development Institute, 331 Shih-Pin Road, Hsinchu, 30062, Taiwan, ROC

    Chien-Hsun Huang, Fwu-Ling Lee & Chun-Ju Tai

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  1. Chien-Hsun Huang
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  2. Fwu-Ling Lee
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  3. Chun-Ju Tai
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Correspondence to Fwu-Ling Lee.

Additional information

The β-tubulin gene sequence data reported in this paper appear in the GenBank nucleotide sequence database with the following accession numbers: FJ238316–FJ238341.

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Huang, CH., Lee, FL. & Tai, CJ. The β-tubulin gene as a molecular phylogenetic marker for classification and discrimination of the Saccharomyces sensu stricto complex. Antonie van Leeuwenhoek 95, 135–142 (2009). https://doi.org/10.1007/s10482-008-9296-1

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