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Ophiostomatoid fungi associated with the northern spruce engraver, Ips perturbatus, in western Canada

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Abstract

A number of ophiostomatoid fungi were isolated from the spruce-infesting bark beetle, Ips perturbatus and its galleries collected from felled spruce trees and logs in northern BC and the Yukon Territory. Isolates were identified to species using morphological characteristics, nuclear ribosomal DNA and partial β-tubulin gene sequences. Thirteen morphological and phylogenetic species were identified among the isolates. Leptographium fruticetum, Leptographium abietinum, Ophiostoma bicolor, Ophiostoma manitobense, O. piceaperdum, and eight undescribed species of the genus Ophiostoma and the anamorph genera Leptographium, Hyalorhinocladiella, Ambrosiella and Graphium. A number of these species, i.e. L. fruticetum, Hyalorhinocladiella sp. 2, O. bicolor and O. manitobense, were isolated repeatedly from I. perturbatus, while others, i.e. Graphium sp. 1 and O. piceaperdum, seemed to be␣sporadic associates. Among all the isolates, L. fruticetum had the highest relative dominance in this survey. A high frequency of occurrence of this species with the beetle may indicate a specific relationship between the two partners.

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Abbreviations

YT:

Yukon Territory

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Acknowledgements

This work was supported by the Natural Sciences and Engineering Research Council of Canada. We would like to thank Mr. Rod Garbutt (Natural Resources Canada, Pacific Forestry Centre, Victoria, BC) and Mike Jull (Aleza Lake Research Forest Society, Prince George, BC) for their help with the field works. We also thank Drs. K. Harrison, J. Reid and G. Hausner for providing cultures.

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  1. Department of Wood Science, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada

    Sepideh Massoumi Alamouti, Jae-Jin Kim & Colette Breuil

  2. Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, Victoria, BC, V8Z 1M5, Canada

    Leland M. Humble

  3. Forintek Canada Corp., 2665 East Mall, Vancouver, BC, V6T 1W5, Canada

    Adnan Uzunovic

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  1. Sepideh Massoumi Alamouti
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Massoumi Alamouti, S., Kim, JJ., Humble, L.M. et al. Ophiostomatoid fungi associated with the northern spruce engraver, Ips perturbatus, in western Canada. Antonie van Leeuwenhoek 91, 19–34 (2007). https://doi.org/10.1007/s10482-006-9092-8

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