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Detection of naturally aerosolized Mycoplasma hyopneumoniae from the air of selected swine farms

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Abstract

We aimed to detect Mycoplasma hyopneumoniae from the air of selected farms through air filtration-based air sampling using polymerase chain reaction (PCR). Air samples were collected at different locations inside and outside of pig farm rooms on polyethersulfone membrane (0.22 μm), and the presence of M. hyopneumoniae DNA was analyzed by PCR. Furthermore, nasal swab and blood samples were collected from 336 pigs in the same air sampled rooms and were analyzed by PCR and IDEXX ELISA, respectively. The suitability of the air sampling method was validated with analysis of an artificially induced aerosolized avirulent M. hyopneumoniae in an enclosed box showing PCR-positive results. M. hyopneumoniae was detected from air sample of pig farm rooms using PCR. Although the probability of an airborne M. hyopneumoniae causing an infection is not yet confirmed, air sampling PCR results could serve as a tool to assess the spread of M. hyopneumoniae by bioaerosols and the infection dynamics in a herd and between herds.

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Acknowledgment

This research was supported in part by Basic Science research Program through the National Research Foundation of Korea (NRF) funded by Ministry of Education, Science and Technology (No. 2011-0021670), Rural Development Administration and by the ministry of Knowledge Economy (MKE) and the Korea Institute for Advancement of Technology 15 (KIAT) through the Center ER Cooperation project. Republic of Korea.

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Authors and Affiliations

  1. College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, South Korea

    Dereje Damte, Sileshi Belew Yohanes, Md. Akil Hossain, Seung-Jin Lee, Man-Hee Rhee & Seung-Chun Park

  2. Gyeongsangbuk-do Veterinary Service Laboratory, Guriro, 702-210, South Korea

    Young-Hoan Kim

Authors
  1. Dereje Damte
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  2. Sileshi Belew Yohanes
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  3. Md. Akil Hossain
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  4. Seung-Jin Lee
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  5. Man-Hee Rhee
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  6. Young-Hoan Kim
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  7. Seung-Chun Park
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Correspondence to Seung-Chun Park.

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Damte, D., Yohanes, S.B., Hossain, M.A. et al. Detection of naturally aerosolized Mycoplasma hyopneumoniae from the air of selected swine farms. Aerobiologia 30, 205–209 (2014). https://doi.org/10.1007/s10453-013-9315-1

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  • DOI: https://doi.org/10.1007/s10453-013-9315-1

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