Apoptotic proteins play key roles in understanding the mechanism of programmed cell death. Knowledge about the subcellular localization of apoptotic protein is constructive in understanding the mechanism of programmed cell death, determining the functional characterization of the protein, screening candidates in drug design, and selecting protein for relevant studies. It is also proclaimed that the information required for determining the subcellular localization of protein resides in their corresponding amino acid sequence. In this work, a new biological feature, class pattern frequency of physiochemical descriptor, was effectively used in accordance with the amino acid composition, protein similarity measure, CTD (composition, translation, and distribution) of physiochemical descriptors, and sequence similarity to predict the subcellular localization of apoptosis protein. AdaBoost with the weak learner as Random-Forest was designed for the five modules and prediction is made based on the weighted voting system. Bench mark dataset of 317 apoptosis proteins were subjected to prediction by our system and the accuracy was found to be 100.0 and 92.4 %, and 90.1 % for self-consistency test, jack-knife test, and tenfold cross validation test respectively, which is 0.9 % higher than that of other existing methods. Beside this, the independent data (N151 and ZW98) set prediction resulted in the accuracy of 90.7 and 87.7 %, respectively. These results show that the protein feature represented by a combined feature vector along with AdaBoost algorithm holds well in effective prediction of subcellular localization of apoptosis proteins. The user friendly web interface “APSLAP” has been constructed, which is freely available at http://apslap.bicpu.edu.in and it is anticipated that this tool will play a significant role in determining the specific role of apoptosis proteins with reliability.
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Saravanan Vijayakumar is supported by the DBT-BINC junior research fellow. The authors thank Borhan Kazimipour of Shiraz University for suggestions in algorithm design. The authors thank Dr. Archana Pan (Centre for Bioinforamtics, Pondicherry University) and Dr. Sivasathya (Department of Computer Science, Ponidcherry University) for their valuable suggestions and P. Elavarasan, E. Malanco and S. Manikandan of Centre for Bioinformatics, Pondicherry University for their technical support in hosting the tool onto the web server.
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Saravanan, V., Lakshmi, P.T.V. APSLAP: An Adaptive Boosting Technique for Predicting Subcellular Localization of Apoptosis Protein. Acta Biotheor 61, 481–497 (2013). https://doi.org/10.1007/s10441-013-9197-1
- Apoptosis protein
- Jack-knife test
- Physio-chemical parameres
- Random forest
- Subcellular localization