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Contribution of Elastic and Collagen Fibers to the Mechanical Behavior of Bovine Nuchal Ligament

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Abstract

Ligamentum nuchae is a highly elastic tissue commonly used to study the structure and mechanics of elastin. This study combines imaging, mechanical testing, and constitutive modeling to examine the structural organization of elastic and collagen fibers and their contributions to the nonlinear stress–strain behavior of the tissue. Rectangular samples of bovine ligamentum nuchae cut in both longitudinal and transverse directions were tested in uniaxial tension. Purified elastin samples were also obtained and tested. It was observed that the stress–stretch response of purified elastin tissue follows a similar curve as the intact tissue initially, but the intact tissue shows a significant stiffening behavior for stretches above 1.29 with collagen engagement. Multiphoton and histology images confirm the elastin-dominated bulk of ligamentum nuchae interspersed with small bundles of collagen fibrils and sporadic collagen-rich regions with cellular components and ground substance. A transversely isotropic constitutive model that considers the longitudinal organization of elastic and collagen fibers was developed to describe the mechanical behavior of both intact and purified elastin tissue under uniaxial tension. These findings shed light on the unique structural and mechanical roles of elastic and collagen fibers in tissue mechanics and may aid in future use of ligamentum nuchae in tissue grafting.

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Acknowledgements

The authors would like to acknowledge the funding support from the National Institute of Health (2R01HL098028).

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  1. Mechanical Engineering, Boston University, 110 Cummington Mall, Boston, MA, 02215, USA

    Samuel Halvorsen, Ruizhi Wang & Yanhang Zhang

  2. Biomedical Engineering, Boston University, Boston, MA, USA

    Yanhang Zhang

  3. Division of Materials Science & Engineering, Boston University, Boston, MA, USA

    Yanhang Zhang

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Halvorsen, S., Wang, R. & Zhang, Y. Contribution of Elastic and Collagen Fibers to the Mechanical Behavior of Bovine Nuchal Ligament. Ann Biomed Eng 51, 2204–2215 (2023). https://doi.org/10.1007/s10439-023-03254-6

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