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Impact of Bi-Axial Shear on Atherogenic Gene Expression by Endothelial Cells

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An Erratum to this article was published on 22 June 2016

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Abstract

This study demonstrated the effects of the directionality of oscillatory wall shear stress (WSS) on proliferation and proatherogenic gene expression (I-CAM, E-Selectin, and IL-6) in the presence of inflammatory mediators leukotriene B4 (LTB4) and bacterial lipopolysaccharide (LPS) from endothelial cells grown in an orbiting culture dish. Computational fluid dynamics (CFD) was applied to quantify the flow in the dish, while an analytical solution representing an extension of Stokes second problem was used for validation. Results indicated that WSS magnitude was relatively constant near the center of the dish and oscillated significantly (0–0.9 Pa) near the side walls. Experiments showed that LTB4 dominated the shear effects on cell proliferation and area. Addition of LPS didn’t change proliferation, but significantly affected cell area. The expression of I-CAM1, E-Selectin and IL-6 were altered by directional oscillatory shear index (DOSI, a measure of the biaxiality of oscillatory shear), but not shear magnitude. The significance of DOSI was further reinforced by the strength of its interactions with other atherogenic factors. Hence, directionality of shear appears to be an important factor in regulating gene expression and provides a potential explanation of the propensity for increased vascular lesions in regions in the arteries with oscillating biaxial flow.

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  • 22 June 2016

    An erratum to this article has been published.

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Author information

Authors and Affiliations

  1. Department of Chemical Engineering, University of Louisville, Ernst Hall 106, Louisville, KY, 40292, USA

    Amlan Chakraborty, Jonathan M. Thomas & R. Eric Berson

  2. Department of Bioinformatics and Biostatistics, University of Louisville, Louisville, KY, USA

    Sutirtha Chakraborty

  3. James Graham Brown Cancer Center and Department of Microbiology & Immunology, University of Louisville, CTRB, Room #324, Louisville, KY, 40202, USA

    Venkatakrishna R. Jala & Bodduluri Haribabu

  4. Department of Mechanical Engineering, University of Louisville, Louisville, KY, USA

    M. Keith Sharp

Authors
  1. Amlan Chakraborty
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  2. Sutirtha Chakraborty
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  3. Venkatakrishna R. Jala
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  4. Jonathan M. Thomas
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  5. M. Keith Sharp
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  6. R. Eric Berson
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  7. Bodduluri Haribabu
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Corresponding authors

Correspondence to R. Eric Berson or Bodduluri Haribabu.

Additional information

Associate Editor Kerry Hourigan oversaw the review of this article.

An erratum to this article is available at https://doi.org/10.1007/s10439-016-1665-8.

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Chakraborty, A., Chakraborty, S., Jala, V.R. et al. Impact of Bi-Axial Shear on Atherogenic Gene Expression by Endothelial Cells. Ann Biomed Eng 44, 3032–3045 (2016). https://doi.org/10.1007/s10439-016-1626-2

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  • DOI: https://doi.org/10.1007/s10439-016-1626-2

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