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Endothelial Cell Micropatterning: Methods, Effects, and Applications

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Abstract

The effects of flow on endothelial cells (ECs) have been widely examined for the ability of fluid shear stress to alter cell morphology and function; however, the effects of EC morphology without flow have only recently been observed. An increase in lithographic techniques in cell culture spurred a corresponding increase in research aiming to confine cell morphology. These studies lead to a better understanding of how morphology and cytoskeletal configuration affect the structure and function of the cells. This review examines EC micropatterning research by exploring both the many alternative methods used to alter EC morphology and the resulting changes in cellular shape and phenotype. Micropatterning induced changes in EC proliferation, apoptosis, cytoskeletal organization, mechanical properties, and cell functionality. Finally, the ways these cellular manipulation techniques have been applied to biomedical engineering research, including angiogenesis, cell migration, and tissue engineering, are discussed.

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Acknowledgments

The authors gratefully acknowledge funding from the American Heart Association grant 09BGIA2260384 and National Institutes of Health grants R01HL103728 and R01HL 095474.

Conflict of Interest

There are no conflicts of interest.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Oregon Health & Science University, CH13B, 3303 SW Bond Ave, Portland, OR, 97239, USA

    Deirdre E. J. Anderson & Monica T. Hinds

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  1. Deirdre E. J. Anderson
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  2. Monica T. Hinds
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Correspondence to Monica T. Hinds.

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Associate Editor Laura Suggs oversaw the review of this article.

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Anderson, D.E.J., Hinds, M.T. Endothelial Cell Micropatterning: Methods, Effects, and Applications. Ann Biomed Eng 39, 2329–2345 (2011). https://doi.org/10.1007/s10439-011-0352-z

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  • DOI: https://doi.org/10.1007/s10439-011-0352-z

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