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Microfluidic platform for human placenta-derived multipotent stem cells culture and applied for enhanced neuronal differentiation

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Abstract

Stem cell research grows rapidly in recent years due to its potential in clinical applications. Since the stem cell behavior is influenced by environmental cues, investigation of the stem cell differentiation under chemical as well as physical stimulation environment is important. Under traditional petri dish culturing conditions, directly applying physical stimulation on the cells is difficult. Microfluidic chip, on the other hand, provides a manageable platform to stimulate cells. In this research, we use microfluidic chip to culture human placenta-derived multipotent stem cells (PDMCs) and apply chemical and physical stimulations to the cells. PDMCs is a newly developed human stem cell source that are easier to access with less ethical issues compare to other human stem cells. This research shows that the microfluidic platform could provide adequate in vitro microenvironment that maintain pluripotency and proliferative potential for PDMCs culture. PDMCs were successfully differentiate to neuronal cells through chemical and physical–chemical stimulation on the microfluidic platform. Our results show that through physical shear stress stimulation, PDMCs can result in enhanced neuronal cell differentiation.

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Acknowledgments

The authors would like to thank the National Science Council, Taiwan (Grant # NSC 102-2221-E-008-041), Cathay General Hospital (Grant # CGH-MR-10120) and National Central University–Cathay General Hospital joint research program (Grant # 101CGHNCU-A2) for financially supporting this project.

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Authors and Affiliations

  1. Proteomics Laboratory, Cathay Medical Research Institute, Cathay General Hospital, Taipei, Taiwan

    Yu-Che Cheng

  2. Institute of Biomedical Engineering, National Central University, Jhongli, Taiwan

    Yu-Che Cheng & Chih-Cheng Chien

  3. Department of Mechanical Engineering, National Central University, 32001, Jhongli, Taiwan

    Chia-Wen Tsao, Meng-Zhi Chiang, Chih-Ang Chung & Chuan Li

  4. Department of Anesthesiology, Sijhih Cathay General Hospital, Sijhih District, New Taipei City, Taiwan

    Chih-Cheng Chien

  5. Department of Chemical and Materials Engineering, National Central University, Jhongli, Taiwan

    Wei-Wen Hu & Ruoh-Chyu Ruaan

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  1. Yu-Che Cheng
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  2. Chia-Wen Tsao
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  3. Meng-Zhi Chiang
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  8. Chuan Li
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Correspondence to Chia-Wen Tsao.

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Cheng, YC., Tsao, CW., Chiang, MZ. et al. Microfluidic platform for human placenta-derived multipotent stem cells culture and applied for enhanced neuronal differentiation. Microfluid Nanofluid 18, 587–598 (2015). https://doi.org/10.1007/s10404-014-1455-2

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  • DOI: https://doi.org/10.1007/s10404-014-1455-2

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