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Japanese Journal of Ophthalmology

, Volume 57, Issue 1, pp 126–132 | Cite as

Renin–angiotensin system involvement in the oxidative stress-induced neurodegeneration of cultured retinal ganglion cells

  • Yoko OzawaEmail author
  • Kenya Yuki
  • Reiko Yamagishi
  • Kazuo Tsubota
  • Makoto Aihara
Laboratory Investigation

Abstract

Purpose

To analyze the influence of oxidative stress on retinal ganglion cells (RGCs) by using a selective culture system of rat RGCs.

Methods

Rat RGCs were purified by a two-step immunopanning procedure and cultured either with or without antioxidant (AO) compounds. Reactive oxygen species (ROS) in RGCs were analyzed using dihydroethidium. Expression of angiotensin II, cleaved caspase 3, and netrin-1 was analyzed by immunocytochemistry. Live RGCs were detected by use of calcein-acetoxymethyl ester. The roles of angiotensin II type 1 receptor (AT1R) signaling and netrin-1 were analyzed by use of an AT1R blocker (telmisartan) and an anti-netrin-1 neutralizing antibody, respectively.

Results

ROS and angiotensin II were induced in RGCs cultured without AO compounds (AO−). In these cultures, the number of live RGCs decreased and expression of cleaved and activated caspase 3 increased, but these changes were attenuated by addition of the AT1R blocker. Reduction in netrin-1 expression under the AO− condition was also prevented by the AT1R blocker. The AT1R blocker’s effects on RGC survival and reduction in cleaved caspase 3-positive cells were cancelled by the anti-netrin-1 neutralizing antibody.

Conclusions

Oxidative stress induced cell death through AT1R signaling and netrin-1 reduction in cultured RGCs.

Keywords

Oxidative stress Angiotensin II Neuroprotection Netrin-1 Retina 

Notes

Acknowledgments

We thank Dr Mao Nakayama, Dr Nyu Rong, and Ms Haruna Koizumi-Mabuchi for technical assistance. The telmisartan was kindly provided by Boehringer Ingelheim, Germany. This study was supported, in part, by a Keio University Gakujishinkoushikin grant to Y.O. The angiotensin II type 1 receptor blocker was a gift from Boehringer Ingelheim, Germany.

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Copyright information

© Japanese Ophthalmological Society 2012

Authors and Affiliations

  • Yoko Ozawa
    • 1
    • 2
    Email author
  • Kenya Yuki
    • 1
    • 2
  • Reiko Yamagishi
    • 3
  • Kazuo Tsubota
    • 2
  • Makoto Aihara
    • 3
  1. 1.Laboratory of Retinal Cell BiologyKeio University School of MedicineTokyoJapan
  2. 2.Department of OphthalmologyKeio University School of MedicineTokyoJapan
  3. 3.Department of Ophthalmology, Graduate School of Medicine and Faculty of MedicineThe University of TokyoTokyoJapan

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