Abstract
Purpose
To evaluate the influence of H2O2 on lens epithelial cells (LECs) and to determine the effect of the Janus kinase (JAK) inhibitor AG490 and the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) kinase (MEK) inhibitor U0126 on LEC death after H2O2 exposure.
Methods
Human lens epithelial (HLE) B-3 cells were cultured. Cells were treated for 45 min with H2O2 and signal transducers and activators transcription (STAT) 3, JAK2, and ERK1/2 phosphorylation were surveyed by Western blot analysis. After pretreatment with either 40 μM AG490 or 25 μM U0126, LECs were exposed to H2O2. LEC death was evaluated by microscopy and flow cytometry.
Results
H2O2 induced phosphorylation of Tyr-705 STAT3, JAK2, and ERK1/2 in LECs. In cells pretreated with both AG490 and U0126, phosphorylation of Tyr-705 STAT3, JAK2, and ERK1/2 was suppressed. Microscopic findings, however, showed that only AG490 noticeably enhanced cell survival, and flow cytometry showed that cell necrosis decreased to 4.05% after pretreatment with 40 μM AG490.
Conclusions
AG490 may prevent H2O2-induced LEC death by blocking an unknown necrosis pathway. Further investigation is required to characterize that pathway.
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Kim, U.S., Nam, SM., Jung, SA. et al. Effects of AG490 on lens epithelial cell death induced by H2O2 . Jpn J Ophthalmol 54, 151–155 (2010). https://doi.org/10.1007/s10384-009-0772-6
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DOI: https://doi.org/10.1007/s10384-009-0772-6