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Expression of c-Jun and Bcl-2 Family Proteins in Apoptotic Photoreceptors of RCS Rats

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Abstract

Purpose

To determine if c-Jun and Bcl-2 family proteins play a role in photoreceptor apoptosis in Royal College of Surgeons (RCS) rats.

Methods

RCS and Sprague-Dawley rats were used. Cryosections of retinas harvested at various postnatal periods were immunostained with antibodies against c-Jun, Bcl-2, and Bax. Double staining with TdT-dUTP nick-end labeling (TUNEL) or propidium iodide (PI) and antibodies was also done. To study the time course of gene and protein expression, semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblotting analyses were carried out.

Results

TUNEL-positive photoreceptors of RCS rats were stained strongly with antibodies against c-Jun and Bax. The number of immunoreactive cells increased on days 21 and 28 after birth (P21 and P28) and decreased on P45. Semiquantitative RT-PCR analysis showed that mRNAs for c-Jun and Bax were upregulated at P21 and P28, but those for Bcl-2 were unchanged. On immunoblotting, a 43-kDa band was revealed by the anti-c-Jun antibody and a 21-kDa band, by the anti-Bax antibody. Protein expression of c-Jun and Bax were increased at both P21 and P28. The temporal profiles of immunoreactivity, protein expression, and mRNA expression were similar.

Conclusion

c-Jun and Bax may play a role in photoreceptor apoptosis in RCS rats. Jpn J Ophthalmol 2006;50:121–127 © Japanese Ophthalmological Society 2006

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Authors and Affiliations

  1. Department of Ophthalmology, Shinshu University School of Medicine, Matsumoto, Japan

    Naomichi Katai, Tomoko Yanagidaira, Nami Senda & Toshinori Murata

  2. Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan

    Nagahisa Yoshimura

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  1. Naomichi Katai
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  2. Tomoko Yanagidaira
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  3. Nami Senda
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  4. Toshinori Murata
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  5. Nagahisa Yoshimura
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Correspondence to Naomichi Katai.

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Katai, N., Yanagidaira, T., Senda, N. et al. Expression of c-Jun and Bcl-2 Family Proteins in Apoptotic Photoreceptors of RCS Rats. Jpn J Ophthalmol 50, 121–127 (2006). https://doi.org/10.1007/s10384-005-0296-7

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  • DOI: https://doi.org/10.1007/s10384-005-0296-7

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