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The Effect of a Hyaluronan-Carboxymethylcellulose Membrane vs. Polyglactin 910 Mesh on Intra-Abdominal Tumor Formation in Mice

  • Original Contribution
  • Published:
Diseases of the Colon & Rectum

Abstract

Purpose

Hyaluronan mediates growth of SW620 colon cancer cells. Because hyaluronan is the active ingredient in Seprafilm®, we hypothesized that Seprafilm® would affect intraperitoneal tumor growth in a mouse model of peritoneal seeding.

Methods

Immunodeficient mice underwent laparotomy and intraperitoneal inoculation of 105 SW620 cells. Seprafilm® (n = 22), Vicryl mesh (foreign body control; n = 24), or no material (sham; n = 19) was placed under the incision. Mice were killed after four weeks and tumors were dissected, counted, and weighed.

Results

Ninety-five percent of mice in the sham group and 96 percent in the Vicryl group developed intraperitoneal tumors. In contrast, only 64 percent of mice in the Seprafilm® group developed tumors (P = 0.024), and these tumors were smaller than those in the sham group; (Seprafilm® = 42 ± 9 mg vs. sham = 82 ± 17 mg; P = 0.05). In contrast, tumors in the Vicryl group were dramatically larger (349 ± 49 mg; P < 0.001 vs. sham or Seprafilm®).

Conclusions

Despite previous data that suggested that hyaluronan increases colon cancer cell growth, we found that Seprafilm® decreased tumor formation and tended to decrease size in this model. In contrast, Vicryl mesh increased tumor formation and size. Our results suggest that Seprafilm® does not promote intraperitoneal tumor growth, especially compared with Vicryl mesh.

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ACKNOWLEDGMENTS

The authors thank Charles LeVea, M.D., Ph.D., Roswell Park Cancer Institute, Buffalo, New York, for pathologic evaluation of the tumors, Mr. Jerry Vincent, University of Minnesota, for technical assistance with Figures 2 and 3, and Genzyme Corporation for their generous gift of Seprafilm®.

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Authors and Affiliations

  1. Department of Surgery, University of Minnesota, Minneapolis, Minnesota

    Peter K. Lee M.D., Andrew P. Windsperger B.A., B.S., Karen R. Wasiluk Ph.D. & David A. Rothenberger M.D.

  2. Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota

    Christopher M. Wilson B.A. & James B. McCarthy Ph.D.

  3. Department of Surgery and Surgical Oncology, Roswell Park Cancer Institute and the University at Buffalo/State University of New York, Buffalo, New York

    Kelli M. Bullard Dunn M.D.

Authors
  1. Peter K. Lee M.D.
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  2. Andrew P. Windsperger B.A., B.S.
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  3. Christopher M. Wilson B.A.
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  4. James B. McCarthy Ph.D.
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  5. Karen R. Wasiluk Ph.D.
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  6. David A. Rothenberger M.D.
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  7. Kelli M. Bullard Dunn M.D.
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Correspondence to Kelli M. Bullard Dunn M.D..

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Lee, P.K., Windsperger, A.P., Wilson, C.M. et al. The Effect of a Hyaluronan-Carboxymethylcellulose Membrane vs. Polyglactin 910 Mesh on Intra-Abdominal Tumor Formation in Mice. Dis Colon Rectum 51, 1403–1407 (2008). https://doi.org/10.1007/s10350-008-9299-z

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  • DOI: https://doi.org/10.1007/s10350-008-9299-z

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