Abstract
PURPOSE: The therapeutic efficacy of lymphocytapheresis in autoimmune diseases is presumed to be caused by depletion of activated lymphocytes. However, nothing is known about the inducer of activated lymphocytes, the antigen-presenting dendritic cells, during lymphocytapheresis. BASIC PROCEDURES: Six sessions of lymphocytapheresis were done in five patients with ulcerative colitis. Dendritic cells were enriched from the buffy coat of depleted lymphocytes and also from the peripheral blood. The phenotype and function of dendritic cells were studied by dual-color flow cytometry and in allogenic mixed leukocyte reaction, respectively. The serum levels of inflammatory cytokines (interleukin-1α 6, 8, 10, and 12 and tumor necrosis factor-α and β) were measured before and after lymphocytapheresis in all cases. MAIN FINDINGS: Lymphocytapheresis was safe and caused clinical, endoscopic or histologic improvements in all patients with ulcerative colitis. Many CD83-positive mature dendritic cells were found in the buffy coats after each session of lymphocytapheresis. The function of dendritic cells, the frequencies of CD83-positive dendritic cells and the levels of interleukin-6 and interleukin-8 were down regulated in all patients with ulcerative colitis after lymphocytapheresis compared with their levels before lymphocytapheresis. CONCLUSIONS: Depletion and down regulation of the function of dendritic cells and diminished levels of inflammatory cytokines caused by lymphocytapheresis may contribute to the therapeutic efficacy of lymphocytapheresis.
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References
M Gotteland M Lopez C Munoz et al. (1999) ArticleTitleLocal and systemic liberation of proinflammatory cytokines in ulcerative colitis Dig Dis Sci 44 830–835
WA Faubion SuffixJr EV Loftus WS Harmsen et al. (2001) ArticleTitleThe natural history of corticosteroid therapy for inflammatory bowel disease Gastroenterology 121 255–260
BE Sands (2000) ArticleTitleTherapy of inflammatory bowel disease Gastroenterology 118 568–582
SC Truelove LJ Witts (1955) ArticleTitleCortisone in ulcerative colitis BMJ 2 1041–1048
K Deusch K Reich (1992) ArticleTitleImmunological aspects of inflammatory bowel disease Endoscopy 24 568–577
S Muller J Lory N Coranza et al. (1998) ArticleTitleActivated CD4+ and CD8+ cytotoxic cells are present in increased numbers in the intestinal mucosa from patients with active inflammatory bowel disease Am J Pathol 152 261–268
YR Mahida K Wu P Jewell (1989) ArticleTitleEnhanced production of interleukin 1 by mononuclear cells isolated from mucosa with active ulcerative colitis and Crohn’s disease Gut 30 835–838
AD Christ AC Stevens H Koeppen et al. (1998) ArticleTitleAn interleukin 12-related cytokine is up-regulated in ulcerative colitis but not in Crohn’s disease Gastroenterology 115 307–313
D Wallace D Goldfinger C Lowe et al. (1982) ArticleTitleA double-blind, controlled study of lympho-plasmapheresis versus sham apheresis in rheumatoid arthritis N Engl J Med 306 1406–1410
FW Miller SF Leitman ME Cronin et al. (1992) ArticleTitleControlled trial of plasma exchange and leukapheresis in polymyositosis and dermatocytosis N Engl J Med 326 1380–1384
E Lerebours A Bussel R Modigliani et al. (1994) ArticleTitleTreatment of Crohn’s disease by lymphocytes apheresis Gastroenterology 107 357–361
T Ayabe T Ashida M Taniguchi et al. (1997) ArticleTitleA pilot study of centrifugal leukocyte apheresis for corticosteroid-resistant active ulcerative colitis Int Med 36 322–327
DG Wright J Karsh AS Fauci et al. (1981) ArticleTitleLymphocyte depletion and immunosuppression with repeated leukapheresis by continuous flow centrifugation Blood 58 451–458
RM Steinman (1991) ArticleTitleThe dendritic cell system and its role in immunogenicity Ann Rev Immunol 9 271–296
J Banchereau RM Steinman (1998) ArticleTitleDendritic cells and the control of immunity Nature 392 245–252
D Bell JW Young J Banchereau (1999) ArticleTitleDendritic cells Adv Immunol 72 255–324
DN Hart (1997) ArticleTitleDendritic cells Blood 90 3245–3287
Y Ikeda SM Akbar H Matsui M Onji (2001) ArticleTitleCharacterization of antigen-presenting dendritic cells in the peripheral blood and in colonic mucosa in patients with ulcerative colitis Eur J Gastroenterol Hepatol 13 841–850
N Romani S Gruner D Brang et al. (1994) ArticleTitleProliferating dendritic cell progenitors in human blood J Exp Med 180 83–93
SG Matts (1961) ArticleTitleThe value of rectal biopsy in the diagnosis of ulcerative colitis Q J Med 30 393–407
K Yamamoto SM Akbar T Masumoto M Onji (1998) ArticleTitleIncreased nitric oxide production by antigen presenting dendritic cells is responsible for low allogenic mixed leukocyte reaction (MLR) in primary biliary cirrhosis Clin Exp Immunol 114 94–101
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Ikeda, Y., Akbar, S.F., Matsui, H. et al. Depletion and Decreased Function of Antigen-Presenting Dendritic Cells Caused by Lymphocytapheresis in Ulcerative colitis. Dis Colon Rectum 46, 521–528 (2003). https://doi.org/10.1007/s10350-004-6593-2
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DOI: https://doi.org/10.1007/s10350-004-6593-2