Abstract
In the study, oxidative stress statuses and antioxidant enzyme activities caused by light were determined by applying two different light intensities to tomato plants under controlled conditions. The indeterminate type Adamset F1 hybrid tomato (Solanum lycopersicum) cultivar was used. It was grown in Hoagland nutrient solution in a controlled climate room with a light/dark photoperiod of 16/8 h, at 27 °C, 65% humidity. The seedlings were grown by applying two different lights of 400 μmol m−1 s−1, which is the optimum light intensity for tomatoes, and 800 μmol m−1 s−1, which is twice the optimum light intensity. In this study, LED lamps, which are close to sunlight, were used as the light source. Sampling for measurement and analysis was done on the 40th day of the application. The Hoagland nutrient solution was replenished every week. The total fresh plant weights (g) of the plants taken on the 40th day and the chlorophyll, malondialdehyde (MDA) amounts and antioxidative enzyme activities of the leaf samples taken from the middle part of the plants were examined. In addition, the amount of MDA and the activities of superoxide dismutase (SOD), catalase (CAT) and ascorbic peroxidase (APX), which are antioxidant enzymes found in the defense system of plants against stress, were determined in order to understand whether oxidative stress occurs. The total fresh weight, chlorophyll and MDA amounts of the plants grown at high light intensity were found to be higher than the optimum light intensity. It has been observed that there is an increase in antioxidative enzyme activities due to the low level of oxidative stress in plants grown at high light intensity. However, it is understood that the resulting stress is at a tolerable level within the antioxidative defense system.
Zusammenfassung
In der Studie wurden die morphologischen und physiologischen Veränderungen bestimmt, die durch die Lichteinwirkung auf Tomatenpflanzen (Solanum lycopersicum) unter kontrollierten Bedingungen mit zwei unterschiedlichen Lichtintensitäten verursacht werden. Verwendet wurde die unbestimmte Tomatensorte Adamset F1 Hybrid (Solanum lycopersicum). Sie wurde in Hoagland-Nährlösung in einem kontrollierten Klimaraum mit einer Licht‑/Dunkel-Photoperiode von 16/8 h bei 27 °C und 65 % Luftfeuchtigkeit gezogen. Die Sämlinge wurden unter zwei verschiedenen Lichtquellen mit 400 μmol m−1 s−1, der optimalen Lichtintensität für Tomaten, und 800 μmol m−1 s−1, der doppelten optimalen Lichtintensität, angebaut. In dieser Studie wurden LED-Lampen, deren Licht ähnlich dem Sonnenlicht ist, als Lichtquelle verwendet. Die Probennahme zur Messung und Analyse erfolgte am 40. Tag der Anwendung. Die Hoagland-Nährlösung wurde jede Woche aufgefüllt. Untersucht wurden die Gesamtpflanzengewichte (g) der am 40. Tag entnommenen Pflanzen sowie die Chlorophyll‑, Malondialdehyd (MDA)-Mengen und antioxidativen Enzymaktivitäten der aus dem mittleren Teil der Pflanzen entnommenen Blattproben. Darüber hinaus wurden die Menge an MDA und die Aktivitäten von Superoxiddismutase (SOD), Katalase (CAT) und Ascorbinsäureperoxidase (APX) bestimmt, um zu verstehen, ob oxidativer Stress auftritt. Es wurde festgestellt, dass das Gesamtgewicht, die Chlorophyll- und MDA-Mengen der Pflanzen, die bei hoher Lichtintensität angebaut wurden, höher waren als die von Pflanzen, die bei optimaler Lichtintensität angebaut wurden. Weiterhin wurde nachgewiesen, dass die antioxidativen Enzymaktivitäten aufgrund des geringen oxidativen Stresses bei Pflanzen, die bei hoher Lichtintensität angebaut werden, zunehmen. Es wird jedoch davon ausgegangen, dass sich der daraus resultierende Stress in einem für das antioxidative Abwehrsystem tolerierbaren Rahmen bewegt.
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Yasar, F., Uzal, O. Oxidative Stress and Antioxidant Enzyme Activities in Tomato (Solanum lycopersicum) Plants Grown at Two Different Light Intensities. Gesunde Pflanzen 75, 479–485 (2023). https://doi.org/10.1007/s10343-022-00716-0
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DOI: https://doi.org/10.1007/s10343-022-00716-0