Abstract
Improving drought tolerance of container seedlings of Japanese larch is of high importance to afforestation. We hypothesized that adequate nitrogen (N) and limited water supply would increase the tolerance of container seedlings to water-deficit stress, circumventing photoinhibition, by means of (i) enhanced photosynthetic capacity with higher leaf N and (ii) decreased water loss from leaves with lower biomass allocation into aboveground parts. Container seedlings of Japanese larch were grown under the treatment combinations of adequate (+ N: 300 mg N container−1) or limited (− N: 150 mg N container−1) N and adequate (+ W: daily irrigation) or limited (− W: twice-a-week irrigation) water. Then, seedlings were subjected to a progressive drought treatment. Higher leaf N was observed in container seedlings grown under + N and − W. During progressive drought, lower stomatal conductance and net photosynthetic rate were observed in leaves with higher leaf N at a given predawn leaf water potential. Furthermore, the maximum efficiency of PSII photochemistry (Fv/Fm) was lower in leaves with higher leaf N, suggesting that higher leaf N might impair intrinsic tolerance to drought at the leaf level contrary to expectations. Conversely, − N and − W seedlings with lower shoot biomass delayed soil drying as a whole-plant response via a reduction in leaf transpiration, leading to delayed photoinhibition as indicated by a decline in Fv/Fm. To circumvent stress at the initial stage of water deficit, lower leaf N via limited N regime and smaller shoot biomass driven by limited N and water regimes would be important.
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Acknowledgements
The authors thank H. Yamamoto for her skillful technical assistance in cultivating the container seedlings and Dr. H. Ito for his valuable advice on statistical analyses. This study was supported in part by Research grant #201605 of the Forestry and Forest Products Research Institute, by the project “Research on development of silviculture system utilizing high performance seedlings and cuttings” funded by Ministry of Agriculture, Forestry and Fisheries of Japan, and by JSPS KAKENHI Grant Number JP17F17102. Evgenios Agathokleous acknowledges multi-year support from The Startup Foundation for Introducing Talent of Nanjing University of Information Science & Technology (NUIST), Nanjing, China (No. 003080). Evgenios Agathokleous was a JSPS International Research Fellow (ID No: P17102). JSPS is a non-profit, independent administrative institution. The authors declare that there are no conflicts of interest.
Funding
This study was supported in part by Research grant #201605 of the Forestry and Forest Products Research Institute, by the project “Research on development of silviculture system utilizing high performance seedlings and cuttings” funded by Ministry of Agriculture, Forestry and Fisheries of Japan and by JSPS KAKENHI Grant Number JP17F17102.
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M.K., E.A. and H.T. designed the study. M.K. and H.H. collected the photosynthetic data; A.U. prepared the seeds of Japanese larch; S.K. and K.Y. collected the leaf N data, performed the analysis and hence equally contributed to this study. All authors also discussed the results and commented on the manuscript. M.K. led the writing with input from E.A. and H.T.
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Kitao, M., Agathokleous, E., Harayama, H. et al. Tolerance of Japanese larch to drought is modified by nitrogen and water regimes during cultivation of container seedlings. Eur J Forest Res 141, 699–712 (2022). https://doi.org/10.1007/s10342-022-01470-8
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DOI: https://doi.org/10.1007/s10342-022-01470-8