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GC-MS Analysis and Evaluation of Antibacterial and Antifungal Activity of Essential Oils Extracted From Fruit Peel of Citrus aurantium L. (Rutaceae) Grown in the West Anatolian Area

GC-MS-Analyse und Beurteilung der bakteriziden und fungiziden Wirkungen von ätherischen Ölen, gewonnen aus Fruchtschalen von Citrus aurantium L. (Rutaceae), angebaut in der Provinz West-Anatolien

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Abstract

In this study, chemical composition and in vitro antibacterial and antifungal effects of essential oils extracted from fruit peel of Citrus aurantium L. grown in West Anatolian ecological conditions, were investigated. Antibacterial and antifungal activities were determined with the use of Minimum Inhibitory Concentration (MIC) method. Essential oils were obtained in a clevenger apparatus and their compositions were analysed by Gas Chromatography-Mass Spectrometry (GC-MS). According to the GC-MS results, dl-limonene (72.51%) and hexasiloxane (13.28%) were the major components in the essential oil obtained from C. aurantium Central population. Limonene (77.27%) and hexasiloxane (6.19%) dominated in the samples originated from Germencik region. Limonene (79.77%) was found to be in the highest concentration in the essential oil obtained from C. aurantium Koçarlı population. Limonene (95.70%) followed by β‑myrcene (0.76%) were the major components of the essential oil obtained from Citrus aurantium Nazilli population. MIC values ranged from 2.5 to 5 µL/mL for gram (−) bacteria and from 0.3125 to 5 µL/mL for gram (+) bacteria. Essential oil from Germencik region generally showed the best effect against fungi. Essential oil obtained from Citrus aurantium fruit peel could be an alternative to synthetic antimicrobial agents.

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Authors and Affiliations

  1. Faculty of Agriculture, Department of Agricultural Biotechnology, Adnan Menderes University, South Campus, Cakmar, Aydin, Turkey

    Emre Sevindik, Kadriye Yıldırım & Gürbüz Palas

  2. Department of Biology, Faculty of Science and Arts, Giresun University, Giresun, Turkey

    Sinem Aydın

  3. Deptartment of Analysis and Food Quality Assessment, University of Life Sciences in Lublin, 8 Skromna St., 20-704, Lublin, Poland

    Monika Sujka

  4. Giresun University, Center Research Laboratory Application and Research Center, Giresun, Turkey

    Elif Apaydın

Authors
  1. Emre Sevindik
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  2. Sinem Aydın
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  3. Monika Sujka
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  4. Elif Apaydın
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  5. Kadriye Yıldırım
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  6. Gürbüz Palas
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Correspondence to Emre Sevindik.

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E. Sevindik, S. Aydın, M. Sujka, E. Apaydın, K. Yıldırım and G. Palas declare that they have no competing interests.

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Sevindik, E., Aydın, S., Sujka, M. et al. GC-MS Analysis and Evaluation of Antibacterial and Antifungal Activity of Essential Oils Extracted From Fruit Peel of Citrus aurantium L. (Rutaceae) Grown in the West Anatolian Area. Erwerbs-Obstbau 63, 135–142 (2021). https://doi.org/10.1007/s10341-021-00549-7

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  • DOI: https://doi.org/10.1007/s10341-021-00549-7

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