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A TaqMan qPCR method for detecting kdr resistance in Aphis gossypii demonstrates improved sensitivity compared to conventional PCR–RFLP

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Abstract

Cotton aphid, Aphis gossypii Glover, has emerged as a prominent pest in Australian cotton production, and monitoring pesticide resistance including pyrethroids in field populations is crucial for its sustainable management. We examined the distribution of kdr resistance in 35 field-collected A. gossypii populations and used TaqMan qPCR assays with pooled samples. The study demonstrated proof of concept that pooled insect qPCR methodology provided effective detection with better sensitivity than individual PCR–RFLP genotyping techniques for the kdr resistance allele. The practical outcome is that routine resistance monitoring can examine more sites while increasing the likelihood of detecting incipient resistance at those sites. More importantly, the method is adaptable to any genetically caused resistance and so not limited to A. gossypii or even insect control. It cannot be overstressed that the ability to detected resistance at very low frequencies is critical to all sustainable resistance management. Early detection of resistance provides critical time for the modification of chemical use prior to potential insecticide control failure.

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Acknowledgments

We thank Brendan Langfield and Kate Marshall for providing technical assistance with the A. gossypii culturing. This study was funded by the Cotton Research and Development Corporation (DAN1203).

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Authors and Affiliations

  1. Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Woodbridge Road, Menangle, NSW, 2568, Australia

    Daniel R. Bogema, Yizhou Chen, Idris M. Barchia & Grant A. Herron

  2. Faculty of Agriculture and Environment, The University of Sydney, Camperdown, NSW, 2006, Australia

    Monica Suann & Sarah Mansfield

Authors
  1. Monica Suann
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  2. Daniel R. Bogema
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  3. Yizhou Chen
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  4. Sarah Mansfield
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  5. Idris M. Barchia
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  6. Grant A. Herron
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Corresponding author

Correspondence to Yizhou Chen.

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Communicated by A. Biondi.

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Suann, M., Bogema, D.R., Chen, Y. et al. A TaqMan qPCR method for detecting kdr resistance in Aphis gossypii demonstrates improved sensitivity compared to conventional PCR–RFLP. J Pest Sci 88, 785–791 (2015). https://doi.org/10.1007/s10340-015-0651-5

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  • DOI: https://doi.org/10.1007/s10340-015-0651-5

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