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Effect of midgut infection with the microsporidium Endoreticulatus schubergi on carbohydrate and lipid levels in Lymantria dispar larvae

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Abstract

Lymantria dispar (Lep., Lymantriidae), an important forest defoliator, is host for a variety of entomopathogenic microsporidia. These are obligate intracellular parasites that completely depend on resources provided by the hosts. The microsporidia infecting L. dispar differ in the host tissues that become infected as well as in virulence, with presumably different effects on host physiology. Endoreticulatus schubergi infects the midgut tissue of the host; its virulence is low. We studied the effects of infection with E. schubergi on carbohydrate and lipid levels in the L. dispar host larva. Trehalose titer in the hemolymph increased with larval age. There were no significant differences between infected and uninfected hosts. Glycogen content of the host tissue also increased with age. Levels of this storage carbohydrate were significantly lower in infected larvae at a late stage of infection when insects were either full grown larvae prior to pupation (57.4 ± 5.7 and 36.4 ± 5.2 μg/mg in uninfected controls and infected hosts, respectively) or prepupae (53.2 ± 3.4 vs. 34.7 ± 4.7 μg/mg). Lipid levels in host tissue likewise increased over time and were slightly but not significantly lower in infected L. dispar at a late stage. The results suggest that L. dispar larvae are able to compensate for most of the loss of nutrients to the developing microsporidia. Differences between these findings and the effects of infection with the virulent microsporidium, Vairimorpha disparis, that infects the host’s fat body and causes severe depletion of nutrients in L. dispar larvae are discussed.

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Acknowledgments

We gratefully acknowledge the support by the Austrian Academy of Sciences for D.K.P., and particularly Prof. Gerhard Glatzel for inviting her as visiting scientist to BOKU University. We thank Andrea Stradner and Gabriele Motlik for technical assistance and Dr. Christa Schafellner for her advice with biochemical analysis and discussions on an earlier draft of this manuscript. Additional funding for this research came from USDA FS cooperative agreement 04-IC-11242343-100.

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Authors and Affiliations

  1. Department of Forest and Soil Sciences, BOKU University of Natural Resources and Applied Life Sciences Vienna, Hasenauerstr. 38, 1190, Vienna, Austria

    Gernot Hoch & Nina Dobart

  2. Institute of Zoology, Bulgarian Academy of Sciences, 1 Tsar Osvoboditel, 1000, Sofia, Bulgaria

    Daniela K. Pilarska

Authors
  1. Gernot Hoch
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  2. Daniela K. Pilarska
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  3. Nina Dobart
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Correspondence to Gernot Hoch.

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Communicated by A. J. Storer.

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Hoch, G., Pilarska, D.K. & Dobart, N. Effect of midgut infection with the microsporidium Endoreticulatus schubergi on carbohydrate and lipid levels in Lymantria dispar larvae. J Pest Sci 82, 351–356 (2009). https://doi.org/10.1007/s10340-009-0260-2

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  • DOI: https://doi.org/10.1007/s10340-009-0260-2

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