Participants
With the expectation of a medium effect size (f = 0.25), power set to 1 − β = 0.95 and an expected correlation among repeated measures of r = 0.4, a priori sample size estimation revealed—for the predicted interaction effect—an optimal sample size of n = 52 (calculated with G*Power 3.1; cf. Faul et al. 2009). However, to compensate for potential dropouts a total of fifty-seven sport-science students were drawn. Five participants had to be excluded from further data analyses because of missing test and or training data. The remaining 25 male (age: M = 21.9 years, SD = 3.3 years) and 27 female (age: M = 20.4 years, SD = 1.2 years) participants had all self-reported normal vision or corrected-to-normal vision by wearing lenses and were right-handed. Balanced by the pretest throwing performance (i.e., radial error, in mm) and the QE data (i.e., QE duration, in ms), the participants were assigned into a high-density vs. low-density training group. All participants received course credits in return and were unaware of the research question. The protocol was approved by the ethics committee of the local Faculty of Human Sciences and was carried out in accordance with the 1964 Declaration of Helsinki.
Apparatus
Participants stood in front of a white screen (width: 3.2 m, height: 2.2 m), on which the target disks (diameter: 125 mm) were projected by a LCD projector (Epson H271B LCD Projector, Nagano, Japan). Ball, head, and hand movement trajectories were collected with a 3D motion-capture system (VICON T20, VICON Motion Systems Limited, Oxford, United Kingdom, 200 Hz). The eye tracker (EyeSeeCam, EyeSeeTec GmbH, Fürstenfeldbruck, Germany, 220 Hz) was integrated in the VICON system and connected via an active optical FireWire extension (GOF-Repeater 800, Unibrain, San Ramon, CA, USA) to a MacBook Pro (Apple, Cupertino, CA, USA), on which the EyeSeeCam software was used for calibrating the eye tracker and streaming eye orientation data over the network. The internal loudspeaker of the main experimental control workstation (HP Z230 Tower-Workstation, Hewlett Packard, Palo Alto, CA, USA) played the audio signals, which were used to structure the timing of the experiments.
Throwing movement and ball flight were assessed by passive retro-reflective markers mounted to a marker cluster (marker diameter: 14 mm) and retro-reflective balls (ball diameter: 50 mm), respectively. The marker cluster was attached to a fingerless glove on the throwing hand by use of velcro tape. The EyeSeeCam assessed the orientation of the left eye by means of an optical tracking of the corneal reflections from infra-red light. The eye orientation data were streamed in real time via Ethernet to the control workstation, which additionally received synchronized positional and rotational head movement data via the retro-reflective markers attached to the EyeSeeCam. With these data, a custom MATLAB (Matlab 2014a, The MathWorks, Natick, MA, USA) software application calculated the three-dimensional gaze vector in the laboratory frame of reference. The accuracy of this integrated eye-tracking system amounts to 0.5° of visual angle with a resolution of 0.01° RMS within 25° of the participant’s field of view (cf. Kredel et al. 2015).
The visual stimuli were programmed in MATLAB 2016b (The Mathworks Natick, MA, USA), and the resulting AVI video files were rendered with Magix Video Pro X3 (Magix Software GmbH, Berlin, Germany) into a MP4 container format with an H.264 compression (resolution: 1280 × 960 px). Data analyses were conducted with MATLAB 2016b, Microsoft Excel 2016 (Microsoft, Redmond, WA, USA), and IBM SPSS Statistics 27 (IBM, Armonk, NY, USA).
Visual stimuli
At the beginning of each trial, either after 1000 ms or after 1300 ms a fixation point was presented either 960 mm off-center to the left or 960 mm off-center to the right with respect to the central point of the screen. After the presentation of an auditory start signal (randomly played between 2200 and 2500 ms after trial start), the target disk was presented horizontally centered at eight different heights, i.e., (from bottom until top) 300 mm (T1), 500 mm (T2), 700 mm (T3), 900 mm (T4), 1300 mm (T5), 1500 mm (T6), 1700 mm (T7), and 1900 mm (T8). Consequently, after the presentation of the fixation point, participants had to saccade to each of the 9 potential target positions. In the test trials, the target always was shown at a height of 1100 mm (TT) (see Fig. 1, left). Each trial lasted 10000 ms with the target disk disappearing after 6000 ms.
Procedure
The experiment was conducted in the institute’s sensorimotor laboratory in individual sessions. After the pretest, participants conducted four learning sessions which were separated by (roughly) seven days. The posttest was conducted directly at the end of the last learning session, and the retention test was delayed by one week.
At the first day, participants received brief instructions and provided informed consent. Subsequently, participants were equipped with the VICON marker cluster glove as well as the EyeSeeCam and were shown an introductory video. The EyeSeeCam was then calibrated, which required participants to consecutively fixate five equidistant points (at 8.5° of visual angle) on the life-sized screen. The EyeSeeCam was re-calibrated if the point of gaze deviated by more than 1° of visual angle from one of the points of the calibration grid, which was checked after every eighth test trial. In each trial, one ball had to be picked out of the ball box positioned at hip height next to the participants. Participants were then asked to fixate the fixation cross until the presentation of the auditory start signal. After the appearance of the target disk, the ball had to be thrown as centrally as possible at the respective target disk with the non-dominant hand using an underhand throwing technique. The participants were centrally positioned in front of the screen at a distance of about 3200 mm (see Fig. 1, right).
On the testing days (pretest, posttest, and retention test), the participants were tested in 2 blocks of 16 trials each at the target TT. The pretest and the retention test were preceded by a 10-trial warm-up block. At the learning days (L1 until L4), participants trained in 10 blocks of 16 trials each. In the high-density group, participants trained predominantly at L1 with the targets T1 and T8 (37.5% of all training trials, each), at L2 with the targets T2 and T7 (37.5% of all training trials, each), at L3 with the targets T3 and T6 (37.5% of all training trials, each), and at L4 with the targets L4 and L5 (37.5% of all training trials, each). At each learning day, the remaining target positions (e.g., targets T2–T7 at L1) were evenly distributed among the remaining training trials. In the low-density group, participants trained the reversed order, thus, at L1 with predominantly T4 and T5 and at L4 with predominantly T1 and T8. Thus, whereas the high-density group experienced increasingly similar action specification, the low-density group experienced increasingly dissimilar action specifications in regard to the target TT. On average, 6.4 days (SD = 1.0 days) elapsed between the pretest and L1, 6.7 days (SD = 0.7 days) between L1 and L2, 7.3 days (SD = 0.9 days) between L2 and L3, exact 7 days between L3 and L4/posttest, and 7.4 days (SD = 0.9 days) between L4/posttest and the retention test. The testing sessions lasted about 30 min and the learning sessions about one hour. At the end of the retention test, the participants were thanked and informed about the aims of the study.
Measures
In pretest, posttest, and retention test, all trials without a valid QE detection and with technical issues (i.e., invalid detection of the ball flight) were removed from further data analyses. Thus, in the pretest on average 17.4% of all trials (SD = 13.3%), in the posttest on average 18.1% of all trials (SD = 11.1%), and in the retention test 16.8% of all trials (SD = 10.4%) could not be analyzed. This means that out of 32 test trials, in the pretest on average 26.4 trials, in the posttest, 26.2 trials, and in the retention test 26.6 trials were used to calculate the dependent measures. Differences in missing trials between training groups were small (pretest: N = 1.0 trials; posttest: N = 0.7 trials, retention test: N = 0.2 trials). The analyses and the results of the training data are reported in Appendix 1.
Quiet Eye
The gaze data were analyzed using the dispersion-based algorithm by Nyström and Holmqvist (2009), which classifies a fixation as soon as the point of gaze becomes stable within a circular area of 1.2° of visual angle for at least 120 ms (for more details, see Kredel et al. 2015). The QE was defined as the final fixation on the target disk before the initiation of the hand’s forward swing. The onset and offset were identified as the first and last VICON frames of the QE fixation, respectively. QE onset and offset were then calculated as relative values in relation to the initiation of the forward swing. Thus, negative values represent moments in time before the initiation of the forward swing, whereas positive values represent moments in time after the initiation of the forward swing. The QE duration was calculated as time interval between QE onset and QE offset. The initiation of the forward swing was determined as the next VICON frame after the average position of the hand reached its local minimum in the sagittal plane before ball release (i.e., one VICON frame after the hand reaching its backmost position; see also Klostermann et al. 2013). QE onset, offset, and duration were separately aggregated for the 3 (test: pretest, posttest, and retention test) times 2 (training group: high-density vs. low-density) factors. Moreover, median splits of QE duration were performed to assess effects of short vs. long QE durations on throwing performance (cf., e.g., Causer et al. 2017; Klostermann 2018).
Throwing performance
Throwing performance was obtained by computing radial-error scores. To this end, the position of the center of the target disk was determined by converting the relative position of the target in the video scene to the physical screen’s frame of reference. The metric deviation of the ball from the target center at ball impact could then be calculated. The throwing performance was separately aggregated for the 3 (test: pretest, posttest, and retention test) times 2 (training group: high-density vs. low-density) factors as well as for long vs. short QE-duration trials.
Statistical analyses
QE duration, QE onset, and QE offset were analyzed with mixed-factorial 3 (test: pretest, posttest, and retention test) times 2 (training group: high-density vs. low-density) ANOVAs with repeated measures on the first factor. In addition, throwing performance was analyzed with mixed-factorial 3 (test: pretest, posttest, and retention test) times 2 (training group: high-density vs. low-density) times 2 (split: long QE duration vs. short QE duration) ANOVAs with repeated measures on the first and the last factors (e.g., Vickers 2016). A posteriori effect sizes were computed as Cohen’s d values and partial eta squared, ηp2. In case of violations of the sphericity assumption, Greenhouse–Geisser corrections were applied.