Abstract
A sensitive and stable spray for thin layer chromatography of acyl-CoA and other thio-compounds has been evaluated. CoA, down to 1 nmol, is readily revealed and by pre- or post-treatment with hydroxylamine or tris(2-carboxyethyl)phosphine, thioesters or disulfides respectively can be stained as well.
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Notes
www.llipid-c.be/specialty-lipids (accessed 10/11/2020).
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S-spray™, used in the initial trials, was a gift from LLipid-C.
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PPVV performed the experiments and wrote the main manuscript.
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The author has recently been approached by LLipid-C to become one of its consultants and hence, depending on the final outcome, might have in the future some financial or non-financial interests. He declares that this did not influence or affect the reported work, being already finalized before this contact.
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Van Veldhoven, P.P. A Stable and Sensitive TLC-Spray for Thiols, Disulfides and Thioesters. Chromatographia (2024). https://doi.org/10.1007/s10337-024-04336-w
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DOI: https://doi.org/10.1007/s10337-024-04336-w