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Simultaneous Determination of Ruscogenin, Neoruscogenin, Trimebutin, and Parabens in Cream Formulation by Reverse Phase High Performance Liquid Chromatography

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Abstract

A simple and stability indicating reverse-phase high-performance liquid chromatographic method for the simultaneous analysis of ruscogenin, neoruscogenin, trimebutine, and parabens including methylparaben and propylparaben has been developed and validated. The separations were performed using a C18 column (250 × 4.6 mm, 5 μm i.d.). The column temperature was set to 25 °C. The mobile phase consisted of acetonitrile (mobile phase B) and 20 mM phosphate buffer pH 3.9 (mobile phase A) and was mixed in gradient elution mode using a binary pump. The developed method was validated according to the requirements of the ICH Q2(R2) guideline. In all cases, the method was found selective for all active substances and parabens. The limit of detection and limit of quantification values ranged from 0.02 to 0.45 µg mL–1 and from 0.06 to 1.35 µg mL–1, respectively. The intraday and interday accuracy (relative error ≤ 1.89) and precision (relative standard deviation ≤ 1.99) values of the method for all compounds were found in the limits. Based on the validation studies, the developed method was found to be selective (peak purity index ≥ 0.9904), sensitive, accurate, precise, and robust. The developed method was successfully applied for the quantification of Ruscogenin, neoruscogenin, trimebutine and methylparaben, propylparaben from the semi-solid dosage form.

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References

  1. Shafik A (2009) Surgical anatomy of hemorrhoids. Surgical treatment of hemorrhoids. Springer, London

    Google Scholar 

  2. Dennison A, Paraskevopoulos J, Kerrigan D, Shorthouse A (1996) Minerva Chir 51:209–216

    CAS  PubMed  Google Scholar 

  3. Lohsiriwat V (2012) World J Gastroenterol WJG 18:2009

    Article  CAS  PubMed  Google Scholar 

  4. Higuero T, Abramowitz L, Castinel A, Fathallah N, Hemery P, Duhoux CL, Pigot F, Pillant-Le Moult H, Senéjoux A, Siproudhis L (2016) J Visc Surg 153:213–218

    Article  CAS  PubMed  Google Scholar 

  5. Ercan G, Tartar RI, Solmaz A, Gulcicek OB, Karagulle OO, Meric S, Cayoren H, Kusaslan R, Kemik A, Kayali DG (2020) Asian J Surg 43:405–416

    Article  PubMed  Google Scholar 

  6. Nazemiyeh H, Zengin G, Mehrad H, Farhoudi M, Bahadori MB (2020) Eur J Integr Med 40:101245

    Article  Google Scholar 

  7. Fu F, Lai Q, Hu J, Zhang L, Zhu X, Kou J, Yu B, Li F (2022) Antioxidants 11:583

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Hua H, Zhu Y, Song Y-H (2018) Biomed Pharmacother 101:115–122

    Article  CAS  PubMed  Google Scholar 

  9. Wu Y, Wang Y, Gong S, Tang J, Zhang J, Li F, Yu B, Zhang Y, Kou J (2020) Biomed Pharmacother 125:109868

    Article  CAS  PubMed  Google Scholar 

  10. He J, Wang Y, Xu L, Xu C, Zhu Y, Xu M, Chen Y, Guo L, Hu W, Xu D (2022) Evid-Based Complement Altern Med 2022:6425121

    Google Scholar 

  11. Wang Y-w, Wu Y-h, Zhang J-z, Tang J-h, Fan R-p, Li F, Yu B-y, Kou J-p, Zhang Y-Y (2021) Acta Pharmacol Sin 42:726–734

    Article  CAS  PubMed  Google Scholar 

  12. Salvioli B (2019) Minerva Gastroenterol Dietol 65:229–238

    Article  PubMed  Google Scholar 

  13. Fan Y-p, Liu P, Xue W-k, Zhao W-j, Pan H-c (2018) Front Pharmacol 9:664

    Article  PubMed  PubMed Central  Google Scholar 

  14. Kountouras J, Gavalas E, Papaefthymiou A, Tsechelidis I, Polyzos SA, Bor S, Diculescu M, Jadallah Κ, Tadeusz M, Karakan T (2020) Medicina 56:339

    Article  PubMed  PubMed Central  Google Scholar 

  15. Nakajima S, Ogawa N, Yokoue N, Tachibana H, Tamada K, Okazawa M, Sato A, Oyama T, Abe H, Kamiya T (2020) Biochem Biophys Res Commun 533:1155–1161

    Article  CAS  PubMed  Google Scholar 

  16. Lee H, Kwon O-B, Lee J-E, Jeon Y-H, Lee D-S, Min S-H, Kim J-W (2021) Cells 10:918

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Güvenç A, Şatır E, Coşkun M (2007) Chromatographia 66:141–145

    Article  Google Scholar 

  18. Nikolov S, Joneidi M, Panova D (1976) Pharmazie 31:611–612

    CAS  PubMed  Google Scholar 

  19. Ding R, Di T-y, Wang G-l, Lin R-c, Liu L-f (2011) Chin J PharmAnal 31:127–130

    CAS  Google Scholar 

  20. Ji P-y, Li Z-w, Yang Q, Wu R (2015) J Chromatogr B 985:71–74

    Article  CAS  Google Scholar 

  21. Zhang H, Xu T, Gao L, Liu X, Liu J, Yu B (2017) Molecules 22:1250

    Article  PubMed  PubMed Central  Google Scholar 

  22. Huang B, Yao C, Bian Q, Wang Z, Mo J (2011) Yao xue xue bao Acta Pharm Sin 46:443–446

    CAS  Google Scholar 

  23. Lavit M, Saivin S, Boudra H, Michel F, Martin A, Cahiez G, Labaune JP, Chomard JM, Houin G (2000) Arzneimittelforschung 50:640–644

    CAS  PubMed  Google Scholar 

  24. Joo E-H, Chang W-I, Oh I, Shin S-C, Na H-K, Lee Y-B (1999) J Chromatogr B Biomed Sci Appl 723:239–246

    Article  CAS  PubMed  Google Scholar 

  25. El-Gindy A, Emara S, Hadad GM (2003) J Pharm Biomed Anal 33:231–241

    Article  CAS  PubMed  Google Scholar 

  26. Zaitsev VN, Syrotchuk OA, Didukh IR (2015) J Chem Pharm Res 7:609–615

    CAS  Google Scholar 

  27. Lin Z-h, Song M (2001) Chin J Pharm Anal 21:25–27

    CAS  Google Scholar 

  28. Wang W, Zuo W-j (2003) Chin J Pharm Anal 23:111–113

    Google Scholar 

  29. Larabi IA, Duverneuil-Mayer C, Abe E, Baud F, Alvarez J-C (2015) J Anal Toxicol 39:720–725

    Article  CAS  PubMed  Google Scholar 

  30. Mostafa AMS, Ahmad AB (1999) Bull Fac Pharm-Cairo Univ 37(3):175–180

    Google Scholar 

  31. Wang H, Zhou H, Horimoto S, Jiang J, Mayumi T, Hu P (2002) J Chromatogr B 779:173–187

    Article  CAS  Google Scholar 

  32. Qin Y, Zhao H, Zhang W, Zhao N, Fan P, Zhang L, Zhang H (2013) Life Sci J 10:2840–2849

    Google Scholar 

  33. Yang Z-d, Zhang Q, Ma B, Hu Y-y, Zheng Y-y, Wang Y-l (2012) Chin J Pharm Anal 32:1351–1356

    CAS  Google Scholar 

  34. Lee S-H, Lee J-H, Cho S, Do S-H, Woo Y-A (2012) Arch Pharmacal Res 35:1599–1607

    Article  CAS  Google Scholar 

  35. Ayad M, El-Balkiny M, Hosny M, Metias Y (2016) Indian J Adv Chem Sci 4:85–97

    CAS  Google Scholar 

  36. Abdel-Wadood HM (2002) Bull Pharm Sci Assiut 25:137–144

    Article  CAS  Google Scholar 

  37. Shaban M (2002) Sci Pharm 70:341–351

    Article  Google Scholar 

  38. Walash M, El Abass SA, Fathy M (2018) Anal Methods 10:4511–4517

    Article  CAS  Google Scholar 

  39. Adhoum N, Monser L (2005) J Pharm Biomed Anal 38:619–623

    Article  CAS  PubMed  Google Scholar 

  40. Li F, Yu L (2001) Biomed Chromatogr 15:248–251

    Article  CAS  PubMed  Google Scholar 

  41. Elqudaby H, Mohamed GG, El Din GM (2014) Int J Electrochem Sci 9:856–869

    Article  Google Scholar 

  42. Elqudaby HM, Mohamed GG, El Din GM (2013) J Pharm Res 7:686–691

    CAS  Google Scholar 

  43. Ayad M, El-Balkiny M, Hosny M, Metias Y (2016) Indian J Adv Chem Sci 4:149–159

    CAS  Google Scholar 

  44. Yu L, Li F (2001) Yao xue xue bao Acta Pharm Sin 36:131–133

    CAS  Google Scholar 

  45. Ali TA, Mohamed GG, El-Sonbati AZ, Diab MA, Elkfass AM (2020) Iran J Pharm Res IJPR 19:533

    CAS  PubMed  Google Scholar 

  46. Rasool N, Kanwal Q, Waseem M, Khan MI (2021) Biomed Chromatogr 35:e4997

    Article  CAS  PubMed  Google Scholar 

  47. Corradini D (2016) Handbook of HPLC. CRC Press

    Book  Google Scholar 

  48. Guideline IHT (2005) Q2 (R1) 1:05

    Google Scholar 

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Funding

No financial support was received from any institution in this study.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Pharmacy, Baskent University, Etimesgut, 06790, Ankara, Turkey

    Gürkan Özen

  2. Department of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University, Sıhhiye, 06100, Ankara, Turkey

    Emirhan Nemutlu

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  1. Gürkan Özen
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  2. Emirhan Nemutlu
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Contributions

GÖ: conceptualization, methodology, formal analysis, writing—original draft, visualization, writing—review & editing. EN: conceptualization, supervision, methodology, visualization, writing—review & editing.

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Correspondence to Gürkan Özen.

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This manuscript has not been submitted to, nor is under review at, another journal or other publishing venue. The authors have no affiliation with any organization with a direct or indirect financial interest in the subject matter discussed in the manuscript.

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Ethical approval is not required as we did not use animals, humans or blood cells in our study.

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Özen, G., Nemutlu, E. Simultaneous Determination of Ruscogenin, Neoruscogenin, Trimebutin, and Parabens in Cream Formulation by Reverse Phase High Performance Liquid Chromatography. Chromatographia 86, 649–657 (2023). https://doi.org/10.1007/s10337-023-04282-z

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  • DOI: https://doi.org/10.1007/s10337-023-04282-z

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