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Simultaneous Determination and Enrichment of β-Sitosterol From Edible Oil Samples Using Poly(NMA-ST-co-TAIC-co-EDMA) Monolith as Sorbent with On-line SPE-HPLC

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Abstract

A poly(NMA-ST-co-TAIC-EDMA) monolithic column was prepared by redox initiation method using N-methylolacrylamide (NMA) and styrene (ST) as co-monomers. The composite monolith was characterized by scanning electron microscopy (SEM) and nitrogen adsorption–desorption isotherm and was used as a solid phase extraction (SPE) absorbent for enrichment of β-sitosterol by high performance liquid chromatography (HPLC). Under the optimum conditions for extraction and determination, the calibration equation was y = 1.02247x + 0.1766; the linear range was 0.015–0.75 mg mL−1 and the linear regression coefficient was 0.998. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.006 mg mL−1 and 0.02 mg mL−1, respectively. The spiked recoveries of β-sitosterol in edible oil samples were 83.60–103.88%; precisions for intra-day and inter-day assays presented as relative standard deviations were less than 6.0% and 6.2%, respectively. The enrichment factor of β-sitosterol was 60 and the results showed that the monolithic column had high selectivity and good permeability as an on-line SPE absorbent for the enrichment and determination of β-sitosterol from edible oils.

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Acknowledgements

The work was supported by the National Natural Science Foundation of China (Nos. 21575033, 21505030), the Natural Science Foundation of Hebei Province (Nos. H2016201221, B2018201270), and the Hebei Province Science and Technology Research Project (grant number QN 2016128).

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Authors and Affiliations

  1. Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding, 071002, China

    Huimin Wang, Bin Guo, Xiaoya Pang, Huan Yu, Haiyan Liu, Huaizhong Guo & Ligai Bai

  2. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Baoding, 071002, China

    Huimin Wang, Bin Guo, Xiaoya Pang, Huan Yu, Haiyan Liu, Hongyuan Yan, Huaizhong Guo & Ligai Bai

  3. College of Public Health, Hebei University, Baoding, 071002, China

    Hongyuan Yan & Dandan Han

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  1. Huimin Wang
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  2. Bin Guo
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  4. Huan Yu
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Correspondence to Haiyan Liu or Ligai Bai.

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Published in the topical collection Recent Trends in Solid-Phase Extraction for Environmental, Food and Biological Sample Preparation with guest editors Anna Laura Capriotti, Giorgia La Barbera, and Susy Piovesana.

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Wang, H., Guo, B., Pang, X. et al. Simultaneous Determination and Enrichment of β-Sitosterol From Edible Oil Samples Using Poly(NMA-ST-co-TAIC-co-EDMA) Monolith as Sorbent with On-line SPE-HPLC. Chromatographia 82, 1285–1293 (2019). https://doi.org/10.1007/s10337-018-3646-6

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  • DOI: https://doi.org/10.1007/s10337-018-3646-6

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