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Evaluation of the Interactions Between Platelets and Alkaloids by Frontal Analysis Capillary Electrophoresis Using Polyvinyl Alcohol-Coated Capillary

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Abstract

Capillary electrophoresis (CE) has been widely used in the study of the interactions between targets (biological macromolecules) and ligands due to its good biocompatibility. However, the separation and analysis of platelets, which play a pivotal role in thrombotic diseases, were limited owing to platelets adhesion in inner wall of capillary during CE analysis. In this paper, polyvinyl alcohol-coated capillaries were simply prepared to prevent the adhesion of platelet. Then, a frontal analysis CE (FACE) method was developed to evaluate the interactions between platelets and eight alkaloids include glaucine, magnoflorine, dehydrocorydaline, palmatine, berberine, isorhynchophylline, rhynchophylline, and brucine. The binding constants and stoichiometries of the interactions were calculated by Scatchard equation for strong and specific interaction, and the non-specific binding equation for weak interaction. The results indicated that glaucine, dehydrocorydaline, and isorhynchophylline showed relatively high affinity interaction with platelets. The developed FACE method may be further applied in the evaluation of interactions between platelets and other small molecular compounds.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (21275169 and 81202886) and the Natural Science Foundation Project of CQ CSTC (cstc2015jcyjA10044), Project no. CQDXWL-2014-Z007 supported by the Fundamental Research Funds for the Central Universities. We gratefully acknowledge the University of Macau for the financial support for this research by the project MYRG2016-00144-ICMS-QRCM.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 401331, People’s Republic of China

    Qiao-Qiao Li, Feng-Qin Wang, Hua Chen, Zhi-Ning Xia & Feng-Qing Yang

  2. Department of Pharmacy, Henan Medical College, Zhengzhou, 451191, People’s Republic of China

    Su-Ying Li

  3. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, People’s Republic of China

    Yuan‑Jia Hu

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  1. Qiao-Qiao Li
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Correspondence to Feng-Qing Yang.

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The authors declared that they have not conflicts of interest.

Ethical approval

All animal experimental procedures were approved by the Institutional Animal Ethical Committee of Chongqing University and were conducted according to the Guide for the Care and Use of Laboratory Animal of the National Institute of Health (Publication no. 80-23, revised 1996).

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Li, QQ., Li, SY., Wang, FQ. et al. Evaluation of the Interactions Between Platelets and Alkaloids by Frontal Analysis Capillary Electrophoresis Using Polyvinyl Alcohol-Coated Capillary. Chromatographia 81, 509–516 (2018). https://doi.org/10.1007/s10337-018-3476-6

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  • DOI: https://doi.org/10.1007/s10337-018-3476-6

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