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Novel Monolithic Stationary Phase with Surface-Grafted Triphenyl Selector for Reversed-Phase Capillary Electrochromatography

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Abstract

A triphenylmethylamine-functionalized monolithic capillary column was newly designed for reversed-phase capillary electrochromatographic applications. Incorporation of the three phenyl rings-containing selector (also referred to as trityl selector) was achieved through post-polymerization functionalization of a generic monolithic matrix bearing nucleophilic-sensitive hydroxysuccinimide moieties. Such a 3D polymer matrix was obtained through UV-induced in situ free radical copolymerization of N-acryloxysuccinimide and ethylene dimethacrylate. The separation properties of the trityl monolithic capillary column were initially evaluated vis-à-vis polycyclic aromatic hydrocarbons, as model hydrophobic compounds, and compared to the separation ability of a benzylamine-functionalized monolithic capillary column prepared using the same generic monolithic matrix. Electrochromatographic separation of phenols and anilines was also considered, and our preliminary results suggest the occurrence of hydrophobic interactions due to the aromatic and non-polar nature of the surface-grafted trityl selector. The triphenyl monolithic capillary column exhibited relative standard deviation values (% RSD) below 4.1 % for the here-studied chromatographic parameters, namely, retention factor, selectivity, resolution, and efficiency.

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Acknowledgments

T.M. is grateful to PROFAS program for financial support during his scientific visit at the University of Paris-East. S.I.K. is grateful to the Ministry of Higher Education and Scientific Research from the Government of Senegal for financial funding during his PhD.

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Authors and Affiliations

  1. Université Paris-Est, ICMPE (UMR7182), CNRS, UPEC, 94320, Thiais, France

    Tahar Mekhalif, Seydina Ibrahima Kebe, Mohamed Guerrouache, Marie Claude Millot & Benjamin Carbonnier

  2. Département de Biologie, Laboratoire de Biochimie Appliquée, Faculté des Sciences, Université Ferhat Abbas, 19000, Sétif, Algeria

    Tahar Mekhalif & Noureddine Belattar

Authors
  1. Tahar Mekhalif
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  2. Seydina Ibrahima Kebe
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  3. Mohamed Guerrouache
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  4. Noureddine Belattar
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  5. Marie Claude Millot
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  6. Benjamin Carbonnier
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Correspondence to Benjamin Carbonnier.

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This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

Tahar Mekhalif declares that he has no conflict of interest. Seydina Ibrahima Kebe declares that he has no conflict of interest. Mohamed Guerrouache declares that he has no conflict of interest. Noureddine Belattar declares that he has no conflict of interest. Marie Claude Millot declares that he has no conflict of interest. Benjamin Carbonnier declares that he has no conflict of interest.

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Mekhalif, T., Kebe, S.I., Guerrouache, M. et al. Novel Monolithic Stationary Phase with Surface-Grafted Triphenyl Selector for Reversed-Phase Capillary Electrochromatography. Chromatographia 79, 1333–1341 (2016). https://doi.org/10.1007/s10337-016-3156-3

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  • DOI: https://doi.org/10.1007/s10337-016-3156-3

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